US 12,228,566 B2
System and method for protein corona sensor array for early detection of diseases
Omid Farokhzad, Waban, MA (US); Morteza Mahmoudi, Brookline, MA (US); and Claudia Corbo, Milan (IT)
Assigned to THE BRIGHAM AND WOMEN'S HOSPITAL, INC., Boston, MA (US)
Filed by THE BRIGHAM AND WOMEN'S HOSPITAL, INC., Boston, MA (US)
Filed on Mar. 15, 2024, as Appl. No. 18/606,701.
Application 17/215,952 is a division of application No. 17/099,331, filed on Nov. 16, 2020, abandoned.
Application 18/606,701 is a continuation of application No. 18/088,946, filed on Dec. 27, 2022, granted, now 12,000,827.
Application 18/088,946 is a continuation of application No. 17/215,952, filed on Mar. 29, 2021, granted, now 11,567,086.
Application 17/099,331 is a continuation in part of application No. 15/880,627, filed on Jan. 26, 2018, granted, now 10,866,242.
Application 15/880,627 is a continuation of application No. PCT/US2017/067013, filed on Dec. 18, 2017.
Claims priority of provisional application 62/435,409, filed on Dec. 16, 2016.
Prior Publication US 2024/0361307 A1, Oct. 31, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/543 (2006.01); C01G 49/02 (2006.01); G01N 33/574 (2006.01); G01N 33/68 (2006.01); G06F 18/24 (2023.01); G06N 20/20 (2019.01); G16B 40/20 (2019.01); B82Y 35/00 (2011.01)
CPC G01N 33/5432 (2013.01) [C01G 49/02 (2013.01); G01N 33/54326 (2013.01); G01N 33/57488 (2013.01); G01N 33/6842 (2013.01); G01N 33/6848 (2013.01); G06F 18/24 (2023.01); G06N 20/20 (2019.01); G16B 40/20 (2019.02); B82Y 35/00 (2013.01); C01P 2004/64 (2013.01); G06F 2218/20 (2023.01)] 20 Claims
OG exemplary drawing
 
1. A method of identifying a pattern of protein biomarkers from a biological fluid, wherein the method comprises:
a. incubating magnetic particles that differ from each other in at least one physicochemical property with the biological fluid from a subject in a disease state to allow biomolecule coronas to form on surfaces of the magnetic particles, and wherein the pattern of protein biomarkers of the biomolecule coronas differs based on the different physicochemical properties of the magnetic particles;
b. isolating the magnetic particles and the biomolecule coronas; and
c. analyzing proteins of the biomolecule coronas by mass spectrometry to identify a relationship between a protein biomarker in the pattern of protein biomarkers and the disease state, wherein the analysis is performed at least in part using a computer.