US 12,228,553 B2
Ultrasmall nanoparticles and methods of making, using and analyzing same
Thomas C. Gardinier, Raritan, NJ (US); Ferdinand F. E. Kohle, Tubingen (DE); Joshua A. Hinckley, Ithaca, NY (US); and Ulrich B. Wiesner, Ithaca, NY (US)
Assigned to Cornell University, Ithaca, NY (US)
Appl. No. 17/052,155
Filed by Cornell University, Ithaca, NY (US)
PCT Filed May 2, 2019, PCT No. PCT/US2019/030492
§ 371(c)(1), (2) Date Oct. 30, 2020,
PCT Pub. No. WO2019/213456, PCT Pub. Date Nov. 7, 2019.
Claims priority of provisional application 62/666,076, filed on May 2, 2018.
Prior Publication US 2021/0048414 A1, Feb. 18, 2021
Int. Cl. G01N 30/02 (2006.01); G01N 21/64 (2006.01); B82Y 30/00 (2011.01); B82Y 35/00 (2011.01); B82Y 40/00 (2011.01)
CPC G01N 30/02 (2013.01) [G01N 21/64 (2013.01); B82Y 30/00 (2013.01); B82Y 35/00 (2013.01); B82Y 40/00 (2013.01); G01N 2021/6439 (2013.01); G01N 2030/027 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of determining the location of one or more dye on and/or in an inorganic nanoparticle comprising a polyethylene glycol layer, comprising subjecting the inorganic nanoparticle or core-shell nanoparticle to high performance liquid chromatography (HPLC) analysis, wherein the method comprises the steps of:
(a) depositing the inorganic nanoparticle in an HPLC column comprising an input in fluid communication with a stationary phase in fluid communication with an output in fluid communication with a detector;
(b) passing a mobile phase through the HPLC column, such that the inorganic nanoparticle elutes from the column and enters the detector, such that the detector generates a signal, wherein the signal indicates the location of the one or more dye on and/or in the inorganic nanoparticle; and
(c) analyzing the signal to determine the location of the one or more dye on and/or in the inorganic nanoparticle.