US 12,228,540 B2
Biochemical test chip
Chen-Yu Yang, Toufen (TW); and Cheng-Yu Chou, Miaoli County (TW)
Assigned to APEX BIOTECHNOLOGY CORP., Hsinchu (TW)
Filed by APEX BIOTECHNOLOGY CORP., Hsinchu (TW)
Filed on Jun. 4, 2021, as Appl. No. 17/339,599.
Claims priority of application No. 109141391 (TW), filed on Nov. 25, 2020.
Prior Publication US 2022/0163476 A1, May 26, 2022
Int. Cl. G01N 27/327 (2006.01); C12Q 1/00 (2006.01)
CPC G01N 27/3272 (2013.01) [C12Q 1/001 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A biochemical test chip, comprising:
an insulating substrate;
an electrode unit, located on the insulating substrate, wherein the electrode unit comprises a working electrode and a counter electrode, wherein a current density of the counter electrode is greater than a current density of the working electrode;
a first insulating septum, located on the electrode unit, wherein the first insulating septum has an opening, and the opening at least partially exposes the electrode unit;
a reactive layer, located at the opening and electrically connected to the electrode unit; and
a second insulating septum, located on the first insulating septum;
wherein the reactive layer and a target analyte undergo a primary reaction, and the counter electrode is configured to undergo a self-redox reaction, wherein the self-redox reaction does not interfere with the primary reaction, and the self-redox reaction allows the counter electrode to have the capability of receiving or releasing additional electrons;
wherein the counter electrode is a cathode, and a standard reduction potential of an active material of the counter electrode satisfies Es0>Em0−Ev, where the Es0 is the standard reduction potential of the active material, the Em0 is a standard reduction potential for concentration reaction on the working electrode, and the Ev is a potential applied by a measuring apparatus when providing measuring reaction.