US 12,228,539 B2
Nanopore-based sequencing with varying voltage stimulus
Roger J. A. Chen, Saratoga, CA (US); J. William Maney, Jr., Emerald Hills, CA (US); and Hui Tian, Cupertino, CA (US)
Assigned to Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on Sep. 7, 2021, as Appl. No. 17/447,048.
Application 17/447,048 is a continuation of application No. 16/719,774, filed on Dec. 18, 2019, granted, now 11,112,375, issued on Sep. 7, 2021.
Application 16/719,774 is a continuation of application No. 15/820,032, filed on Nov. 21, 2017, granted, now 10,545,112, issued on Jan. 28, 2020.
Application 15/820,032 is a continuation of application No. 14/971,667, filed on Dec. 16, 2015, granted, now 9,863,904, issued on Jan. 9, 2018.
Application 14/971,667 is a continuation in part of application No. 14/577,511, filed on Dec. 19, 2014, granted, now 9,557,294, issued on Jan. 31, 2017.
Prior Publication US 2021/0396701 A1, Dec. 23, 2021
Int. Cl. G01N 27/22 (2006.01); C12Q 1/6869 (2018.01); G01N 27/447 (2006.01); G01N 33/487 (2006.01)
CPC G01N 27/228 (2013.01) [C12Q 1/6869 (2013.01); G01N 27/44791 (2013.01); G01N 33/48721 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A method for analyzing a molecule in a nanopore, the method comprising:
passing a portion of the molecule through the nanopore in a membrane;
determining a rate of voltage decay across a capacitor in electrical communication with the nanopore as the portion of the molecule passes through the nanopore; and
identifying the portion of the molecule from a predetermined selection of molecules based on the determined rate of voltage decay.