| CPC G01N 21/359 (2013.01) [A61B 5/14542 (2013.01); A61B 5/1455 (2013.01); A61B 5/6814 (2013.01); G01N 21/49 (2013.01); A61B 5/0077 (2013.01); A61B 5/14546 (2013.01); A61B 5/1495 (2013.01); G01N 21/274 (2013.01)] | 16 Claims |

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1. A method of non-invasively determining a blood circulatory hemoglobin value for a subject using a near-infrared spectrophotometric (NIRS) sensing device, comprising:
non-invasively sensing tissue of the subject using a NIRS sensing device during a period of time, and determining at least one NIRS tissue total hemoglobin (totalHb) value based on the non-invasive sensing during the period of time;
inputting at least one blood circulatory total hemoglobin (THb) value representative of the subject's blood circulatory THb during the period of time into the NIRS sensing device, wherein the NIRS sensing device is configured to self-calibrate using the at least one blood circulatory THb value and the at least one NIRS tissue totalHb value; and
determining at least one blood circulatory hemoglobin value using the calibrated NIRS sensing device and at least one subsequent NIRS tissue totalHb value;
wherein the NIRS sensing devices uses stored empirical data during self-calibration, said stored empirical data including an empirical circulatory THb calibration slope.
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