US 12,228,496 B2
Apparatus and method for cell particle sorting based on microfluidic-chip flows
Myung-Suk Chun, Seoul (KR); Sun Mi Lee, Seoul (KR); Jae Hun Kim, Seoul (KR); and Chansung Park, Seoul (KR)
Assigned to KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed by KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed on Oct. 29, 2020, as Appl. No. 17/083,299.
Claims priority of application No. 10-2020-0132871 (KR), filed on Oct. 14, 2020.
Prior Publication US 2022/0113243 A1, Apr. 14, 2022
Int. Cl. G01N 15/14 (2024.01); B01L 3/00 (2006.01); G01N 21/64 (2006.01)
CPC G01N 15/1484 (2013.01) [B01L 3/502761 (2013.01); G01N 15/147 (2013.01); G01N 21/64 (2013.01); B01L 2200/0652 (2013.01); B01L 2400/0478 (2013.01); G01N 2015/1472 (2013.01)] 16 Claims
OG exemplary drawing
 
1. An apparatus for cell particle sorting based on microfluidic-chip flow, the apparatus comprising:
a spiral channel structure coupled with a double hydrodynamic filtration channel having different cut-off width (WC) values under Dean number (De) conditions;
the spiral channel structure comprising a first substrate configured such that Dean flow focusing occurs;
the double hydrodynamic filtration channel comprising a second substrate configured such that hydrodynamic filtration (HDF) occurs in parallel among a first HDF channel and a second HDF channel having different WC values; and
an inter-substrate way connecting the first substrate to the second substrate,
wherein at least a part of a sample solution of cell particles moves to the second substrate after passing through the first substrate,
wherein the inter-substrate way comprises two or more paths between the first substrate and the second substrate, each path selecting particles of the sample solution of cell particles according to a value of De determined by flow conditions of the sample solution flowing into the first substrate, and
wherein a first path of the two or more paths couples a spiral inner-outlet of the first substrate to the first HDF channel of the second substrate and a second path of the two or more paths couples a spiral outer-outlet of the first substrate to the second HDF channel of the second substrate.