US 12,228,494 B2
Pressure regulated fluidics system for flow cytometers
David Vrane, Fremont, CA (US)
Assigned to Cytek Biosciences, Inc., Fremont, CA (US)
Filed by Cytek Biosciences, Inc., Fremont, CA (US)
Filed on Apr. 7, 2024, as Appl. No. 18/628,729.
Application 18/628,729 is a continuation of application No. 17/390,984, filed on Aug. 1, 2021, granted, now 11,965,813.
Claims priority of provisional application 63/060,663, filed on Aug. 3, 2020.
Prior Publication US 2024/0280464 A1, Aug. 22, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 15/14 (2024.01); G01N 15/1404 (2024.01); G01N 15/10 (2006.01); G01N 15/1409 (2024.01)
CPC G01N 15/1404 (2013.01) [G01N 2015/1006 (2013.01); G01N 15/1409 (2024.01)] 9 Claims
OG exemplary drawing
 
1. A pressure regulated fluidics system of a flow cytometer, the pressure regulated fluidics system comprising:
a sheath tank holding sheath fluid;
a degasser coupled to the sheath tank by a first sheath line, the degasser to pull gas molecules out of the sheath fluid;
a manifold assembly coupled to the degasser by a second sheath line;
a first valve coupled to the second sheath line;
a pressure regulator coupled to the sheath tank, the pressure regulator configured to apply a regulated pressure to the sheath tank and the sheath fluid therein to maintain a total flow rate of fluids through the flow cytometer; and
a transducer coupled to and between the pressure regulator and the first valve, wherein the transducer is configured to sense measured pressure at the first valve and convert the measured pressure into a voltage;
wherein the pressure regulator is configured to receive the voltage from the transducer to apply the regulated pressure in an attempt to match the regulated pressure at the sheath tank with the measured pressure at the manifold assembly.