US 12,228,492 B2
Compositions and methods for cell-like calibration particles
Jeffrey Kim, Berkeley, CA (US); Anh Tuan Nguyen, San Francisco, CA (US); and Brandon Miller, Oakland, CA (US)
Assigned to Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed by Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed on Jun. 3, 2024, as Appl. No. 18/731,774.
Application 18/731,774 is a continuation of application No. 18/213,934, filed on Jun. 26, 2023, granted, now 12,038,369.
Application 18/213,934 is a continuation of application No. 17/727,879, filed on Apr. 25, 2022, granted, now 11,726,023, issued on Aug. 15, 2023.
Application 17/727,879 is a continuation of application No. 17/155,294, filed on Jan. 22, 2021, granted, now 11,313,782, issued on Apr. 26, 2022.
Claims priority of provisional application 62/965,494, filed on Jan. 24, 2020.
Prior Publication US 2024/0319067 A1, Sep. 26, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 15/10 (2024.01)
CPC G01N 15/1012 (2013.01) [G01N 2015/1006 (2013.01); G01N 2015/1014 (2024.01)] 30 Claims
OG exemplary drawing
 
1. A composition comprising: a hydrogel particle having
a) a polymer matrix; and
b) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell;
wherein the hydrogel particle is configured to bind to an antibody that is bound to a conjugated fluorophore.