	US 12,228,482 B2
	Devices and methods for overlaying blood or cellular suspensions
Jae Go Kwon, Thousand Oaks, CA (US)
Filed by Jae Go Kwon, Thousand Oaks, CA (US)
Filed on Aug. 17, 2021, as Appl. No. 17/404,647.
Application 17/404,647 is a continuation of application No. 16/532,049, filed on Aug. 5, 2019, granted, now 11,105,718.
Application 16/532,049 is a continuation of application No. 14/237,516, granted, now 10,371,609, issued on Aug. 6, 2019, previously published as PCT/US2012/050192, filed on Aug. 9, 2012.
Claims priority of provisional application 61/521,573, filed on Aug. 9, 2011.
Prior Publication US 2021/0372891 A1, Dec. 2, 2021
Int. Cl. G01N 1/31 (2006.01); B01L 3/00 (2006.01); B01L 3/02 (2006.01)

CPC G01N 1/31 (2013.01) [B01L 3/5021 (2013.01); B01L 3/0293 (2013.01); B01L 2200/026 (2013.01); B01L 2200/0642 (2013.01); B01L 2300/046 (2013.01); B01L 2400/0457 (2013.01); B01L 2400/086 (2013.01)]

17 Claims

1. A system, comprising:

an open-ended container having an inner surface extending from a top end to a bottom end; and

a fluid layering device disposed in the open-ended container nearer to the bottom end than the top end, wherein the fluid layering device is configured to control a flow of fluid to the bottom end of the open-ended container, the fluid layering device comprising a trap member including a cylindrical wall opposing the inner surface of the open-ended container and a conical wall tapering downward from an upper edge of the cylindrical wall to a central opening.

7. A method for overlaying blood or cellular suspension over a volume of density gradient contained in a container having an open top end, a closed bottom end, and an inner surface extending from the open top end to the closed bottom end, the method comprising:

inserting the volume of density gradient into the open top end of the container;

inserting a fluid layering device into the container such that the fluid layering device is disposed in the container nearer to the closed bottom end than the open top end, wherein the fluid layering device is configured to control a flow of fluid to the closed bottom end of the container, the device comprising a trap member including a cylindrical wall opposing the inner surface of the container and a conical wall tapering downward from an upper edge of the cylindrical wall to a central opening; and

inserting a blood or cellular suspension into the open top end of the container.