US 12,228,480 B2
Biological sample preparation using electric fields
Michael Nilsson, Mendon, MA (US); and Erhard Ralf Schoenbrunner, Charlestown, MA (US)
Assigned to Revvity Health Sciences, Inc., Waltham, MA (US)
Filed by Revvity Health Sciences, Inc., Waltham, MA (US)
Filed on Nov. 26, 2019, as Appl. No. 16/696,269.
Claims priority of provisional application 62/773,927, filed on Nov. 30, 2018.
Prior Publication US 2020/0173890 A1, Jun. 4, 2020
Int. Cl. G01N 1/28 (2006.01); G01N 1/40 (2006.01); G01N 27/327 (2006.01); G01N 27/414 (2006.01); G01N 33/483 (2006.01); G01N 33/487 (2006.01)
CPC G01N 1/2813 (2013.01) [G01N 1/40 (2013.01); G01N 27/3275 (2013.01); G01N 27/4145 (2013.01); G01N 33/4836 (2013.01); G01N 33/48721 (2013.01); G01N 2001/2826 (2013.01); G01N 2001/4038 (2013.01)] 31 Claims
OG exemplary drawing
 
1. A method for separating bioanalytes from a substrate comprising a biological sample, comprising the steps of:
submerging at least a portion of the substrate containing the biological sample in a buffer solution, wherein the biological sample is blood and the substrate is a filter paper, the substrate having an end face spotted with the biological sample and a side surface joined to the end face;
bringing a first elongated electrical conductor into contact with the buffer solution, wherein the first elongated electrical conductor is a positive electrode;
bringing a second elongated electrical conductor into contact with the buffer solution, wherein the second elongated electrical conductor is a negative electrode, the side surface of the substrate faces at least one of the first elongated electrical conductor and the second elongated electrical conductor, and the end face of the substrate spotted with the biological sample faces a direction that is perpendicular to a side surface of at least one of the first and second elongated electrical conductors;
creating a difference in electric potential energy between the first and second elongated electrical conductors; and
collecting separated bioanalytes from at least one of the first and second elongated electrical conductors.