US 12,227,812 B2
Compositions and methods for detecting nucleic acids of Epstein-Barr virus
Ankur Shah, San Diego, CA (US); and Jian Yu Fung, Chino Hills, CA (US)
Assigned to Gen-Probe Incorporated, San Diego, CA (US)
Appl. No. 17/264,574
Filed by GEN-PROBE INCORPORATED, San Diego, CA (US)
PCT Filed Aug. 1, 2019, PCT No. PCT/US2019/044614
§ 371(c)(1), (2) Date Jan. 29, 2021,
PCT Pub. No. WO2020/028631, PCT Pub. Date Feb. 6, 2020.
Claims priority of provisional application 62/713,330, filed on Aug. 1, 2018.
Prior Publication US 2021/0332449 A1, Oct. 28, 2021
Int. Cl. C12Q 1/70 (2006.01)
CPC C12Q 1/705 (2013.01) [C12Q 2600/158 (2013.01)] 44 Claims
 
1. A reaction mixture for determining the presence or absence of Epstein-Barr virus (EBV) in a sample, said reaction mixture comprising:
a detection probe oligomer for detecting EBV nucleic acids,
wherein the detection probe oligomer is up to 30 nucleotides in length and comprises the base sequence of SEQ ID NO:22 or the complement thereof, allowing for substitution of RNA and DNA equivalent bases; and
a pair of amplification oligomers,
wherein a first amplification oligomer of the pair comprises 18-25 contiguous bases of SEQ ID NO:3, allowing for substitution of RNA and DNA equivalent bases, and
wherein a second amplification oligomer of the pair comprises 18-25 contiguous bases of SEQ ID NO:4, allowing for substitution of RNA and DNA equivalent bases.