US 12,227,801 B2
Methods, systems, and compositions for nucelic acid sequencing
Suhua Deng, Palo Alto, CA (US); Vladimir Ivanovich Bashkirov, Davis, CA (US); Hui Tian, Cupertino, CA (US); and Igor Constantin Ivanov, Danville, CA (US)
Assigned to AXBIO INC., Santa Clara, CA (US)
Filed by AXBIO INC., Santa Clara, CA (US)
Filed on Feb. 3, 2023, as Appl. No. 18/164,102.
Application 18/164,102 is a continuation of application No. 16/953,667, filed on Nov. 20, 2020, granted, now 11,603,562.
Application 16/953,667 is a continuation of application No. PCT/US2019/033376, filed on May 21, 2019.
Claims priority of provisional application 62/674,706, filed on May 22, 2018.
Prior Publication US 2024/0076730 A1, Mar. 7, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6806 (2013.01)] 20 Claims
 
1. A method for processing or analyzing a nucleic acid molecule, comprising:
(a) providing (i) said nucleic acid molecule and (ii) a double-stranded adapter having a nick within a strand of said double-stranded adapter;
(b) coupling said double-stranded adapter to said nucleic acid molecule;
(c) circularizing said nucleic acid molecule coupled to said double-stranded adapter to generate a circularized nucleic acid molecule, wherein said circularized nucleic acid molecule comprises a first strand and a second strand, wherein said first strand comprises said nick;
(d) contacting said circularized nucleic acid molecule with a tagged nucleotide under conditions sufficient to couple said tagged nucleotide to an end of said first strand of said circularized nucleic acid molecule, and thereby generating a growing strand, wherein said tagged nucleotide comprises a nucleotide coupled to a tag, wherein said growing strand has sequence complementarity to at least a portion of said second strand of said circularized nucleic acid molecule; and
(e) obtaining sequence information of at least a portion of said growing strand during said generating in (d).