US 12,227,798 B2
Methods and compositions for incorporating nucleotides
Steven Gordon, Weston, MA (US); and Jerzy Olejnik, Brookline, MA (US)
Assigned to IsoPlexis Corporation, Branford, CT (US)
Filed by IsoPlexis Corporation, Branford, CT (US)
Filed on Mar. 30, 2021, as Appl. No. 17/217,354.
Application 17/217,354 is a continuation of application No. 16/430,064, filed on Jun. 3, 2019, granted, now 11,001,887, issued on May 11, 2021.
Application 16/430,064 is a continuation of application No. 15/911,801, filed on Mar. 5, 2018, granted, now 10,329,611, issued on Jun. 25, 2019.
Application 15/911,801 is a continuation of application No. 15/214,737, filed on Jul. 20, 2016, granted, now 9,909,174, issued on Mar. 6, 2018.
Application 15/214,737 is a continuation of application No. 14/691,042, filed on Apr. 20, 2015, granted, now 9,434,989, issued on Sep. 6, 2016.
Application 14/691,042 is a continuation of application No. 13/305,415, filed on Nov. 28, 2011, granted, now 9,017,973, issued on Apr. 28, 2015.
Application 13/305,415 is a continuation of application No. 12/405,779, filed on Mar. 17, 2009, abandoned.
Claims priority of provisional application 61/037,845, filed on Mar. 19, 2008.
Prior Publication US 2021/0230686 A1, Jul. 29, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C07H 19/00 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01); G01N 21/05 (2006.01); G01N 21/64 (2006.01); G01N 21/03 (2006.01)
CPC C12Q 1/6869 (2013.01) [C07H 19/00 (2013.01); C12Q 1/6874 (2013.01); G01N 21/05 (2013.01); G01N 21/6428 (2013.01); G01N 21/645 (2013.01); C12Q 2535/101 (2013.01); G01N 2021/0346 (2013.01); G01N 2021/058 (2013.01); G01N 2021/6417 (2013.01); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/6441 (2013.01); G01N 2021/6471 (2013.01); G01N 2201/062 (2013.01); G01N 2201/0627 (2013.01); G01N 2201/12 (2013.01)] 8 Claims
 
1. A method for determining an identity of a nucleotide at a position, comprising:
a) providing nucleic acid, polymerase, and a mixture of labeled and non-labeled reversible terminators, said labeled reversible terminators comprising a label linked to a nucleotide analogue;
b) exposing said nucleic acid, said polymerase, and said mixture to extension conditions for 10 minutes or less, wherein a first nucleotide analogue from said mixture is incorporated into said nucleic acid at a position;
c) detecting the label of said incorporated first nucleotide analogue; and
d) determining the identity of the incorporated nucleotide at said position.