US 12,227,797 B2
Spatial analysis of a planar biological sample
Olof John Ericsson, Saltsjöbaden (SE)
Assigned to MOLECULENT AB, Solna (SE)
Appl. No. 18/569,594
Filed by MOLECULENT AB, Saltsjöbaden (SE)
PCT Filed Jun. 23, 2022, PCT No. PCT/IB2022/055849
§ 371(c)(1), (2) Date Dec. 12, 2023,
PCT Pub. No. WO2022/269543, PCT Pub. Date Dec. 29, 2022.
Claims priority of provisional application 63/257,456, filed on Oct. 19, 2021.
Claims priority of provisional application 63/214,701, filed on Jun. 24, 2021.
Prior Publication US 2024/0287587 A1, Aug. 29, 2024
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6841 (2018.01)
CPC C12Q 1/6841 (2013.01) [C12Q 1/6806 (2013.01)] 22 Claims
OG exemplary drawing
 
1. An in vitro method for analyzing a sample, comprising:
(a) contacting an oligonucleotide or a conjugate comprising the same with a planar biological sample that is a tissue section under conditions by which the oligonucleotide or conjugate specifically binds to sites in or on the tissue section;
(b) performing one or more steps to release and/or extend the oligonucleotide in situ, to produce a reporter probe;
(c) transferring all or a part of the reporter probe from the tissue section to a planar support that does not comprise oligonucleotides, wherein the transferring is done by placing the tissue section on the support and transferring the reporter probe onto the surface of the support by diffusion such that the spatial relationship of the reporter probe in the tissue section is preserved, wherein the planar support is glass; and
(d) detecting the reporter probe on the glass support by microscopy,
wherein step (d) comprises hybridizing one or more labeled oligonucleotides, directly or indirectly, to the reporter probe and then detecting the binding pattern of the labeled oligonucleotides by microscopy, and
wherein the method is free of any nucleic acid amplification on the planar support.