US 12,227,789 B2
Methods, compositions, and devices for isolation and expression analysis of regions of interest from a tissue
John Butler, San Jose, CA (US); and Bidhan Chaudhuri, San Jose, CA (US)
Assigned to QUANTUMCYTE, INC., Sunnyvale, CA (US)
Filed by Quantumcyte, Inc., Sunnyvale, CA (US)
Filed on Sep. 2, 2020, as Appl. No. 17/010,390.
Application 17/010,390 is a continuation of application No. PCT/US2019/020610, filed on Mar. 4, 2019.
Claims priority of provisional application 62/691,559, filed on Jun. 28, 2018.
Claims priority of provisional application 62/637,998, filed on Mar. 2, 2018.
Prior Publication US 2021/0222229 A1, Jul. 22, 2021
Int. Cl. C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01); G01N 1/06 (2006.01)
CPC C12Q 1/6806 (2013.01) [C12Q 1/6869 (2013.01); G01N 1/06 (2013.01)] 28 Claims
OG exemplary drawing
 
1. A method of isolating cellular components from at least one region of interest within a planar tissue section disposed onto a solid substrate, comprising:
(a) circumscribing at least one region of interest within the planar tissue section disposed onto the solid substrate by applying a chemical mask to the planar tissue section;
(b) solubilizing the cellular components within the at least one region of interest by dispensing a first solution comprising one or more extraction agents into the at least one region of interest whereby cells circumscribed within the at least one region of interest are selectively lysed, and a convex liquid dome is formed within each region of the at least one region of interest wherein the convex liquid dome is isolated from a fluid communication with other convex liquid domes in other regions of interest of the at least one region of interest and the other convex liquid domes are isolated from a fluid communication with each other.