US 12,227,785 B2
Compositions comprising a low pH alpha-amylase and a thermostable protease for liquefaction
Randy Deinhammer, Wake Forest, NC (US); Suzanne Clark, Youngsville, NC (US); Mauricio Quiros, Raleigh, NC (US); John Matthews, Louisburg, NC (US); Anne Glud Hjulmand, Raleigh, NC (US); Chee-Leong Soong, Raleigh, NC (US); Tomoko Matsui, Chiba (JP); and Shinobu Takagi, Chiba (JP)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Filed by NOVOZYMES A/S, Bagsvaerd (DK)
Filed on Oct. 23, 2023, as Appl. No. 18/492,525.
Application 18/492,525 is a continuation of application No. 18/052,080, filed on Nov. 2, 2022, granted, now 11,840,718.
Application 18/052,080 is a continuation of application No. 17/036,601, filed on Sep. 29, 2020, granted, now 11,566,266.
Application 17/036,601 is a continuation of application No. 16/407,840, filed on May 9, 2019, granted, now 10,941,422.
Application 16/407,840 is a continuation of application No. 15/727,952, filed on Oct. 9, 2017, granted, now 10,947,567.
Application 15/727,952 is a continuation of application No. 13/994,310, granted, now 9,816,112, previously published as PCT/US2011/066559, filed on Dec. 21, 2011.
Application 18/052,080 is a continuation of application No. 17/072,557, filed on Oct. 16, 2020, granted, now 11,499,170, issued on Nov. 15, 2022.
Claims priority of provisional application 61/566,373, filed on Dec. 2, 2011.
Claims priority of provisional application 61/426,039, filed on Dec. 22, 2010.
Prior Publication US 2024/0102056 A1, Mar. 28, 2024
Prior Publication US 2025/0002948 A9, Jan. 2, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/06 (2006.01); C12P 19/02 (2006.01); C12P 19/14 (2006.01); C12P 19/20 (2006.01)
CPC C12P 7/06 (2013.01) [C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); C12Y 304/24 (2013.01); C12P 19/02 (2013.01); C12P 19/14 (2013.01); C12P 19/20 (2013.01); Y02E 50/10 (2013.01)] 21 Claims
 
1. A composition comprising an alpha-amylase and a protease, wherein i) the alpha-amylase has at least 85% identity, but less than 100% identity, to the polypeptide of SEQ ID NO: 1 and has a T½ (min) at pH 4.5, 85 degrees centigrade, 0.12 mM CaCl2 of at least 10; and
ii) the protease has at least 85% identity, but less than 100% identity, to the polypeptide of SEQ ID NO: 13, wherein the protease has a thermostability value of more than 50 percent determined as Relative Activity at 80 degrees centigrade/70 degrees centigrade.