	US 12,227,782 B2
	Method for producing protein
Nozomu Shibata, Wakayama (JP); and Toshiharu Arai, Wakayama (JP)
Assigned to Kao Corporation, Tokyo (JP)
Appl. No. 17/252,010
Filed by Kao Corporation, Tokyo (JP)
PCT Filed Jun. 27, 2019, PCT No. PCT/JP2019/025548 § 371(c)(1), (2) Date Dec. 14, 2020, PCT Pub. No. WO2020/008988, PCT Pub. Date Jan. 9, 2020.
Claims priority of application No. 2018-127519 (JP), filed on Jul. 4, 2018; and application No. 2018-211690 (JP), filed on Nov. 9, 2018.
Prior Publication US 2021/0254117 A1, Aug. 19, 2021
Int. Cl. C12N 1/38 (2006.01); C12N 1/14 (2006.01); C12N 9/24 (2006.01); C12P 21/00 (2006.01)

CPC C12P 21/00 (2013.01) [C12N 1/14 (2013.01); C12N 9/2402 (2013.01); C12Y 302/01004 (2013.01); C12N 2500/34 (2013.01); C12N 2500/60 (2013.01)]

14 Claims

1. A method for producing protein, comprising culturing a microorganism in the presence of glycosylamine,

wherein the microorganism is a Trichoderma fungus,

wherein the glycosylamine is at least one selected from the group consisting of β-D-glucopyranosylamine, β-D-mannopyranosylamine and β-D-galactopyranosylamine,

wherein the glycosylamine is continuously fed to the culture during the culturing, and the amount of the glycosylamine that is fed is 5 g/hr or less per liter (L) of an initial culture medium, and

wherein, in the method for producing protein, productivity of the protein is increased as a result of the culturing as compared to productivity of the protein when the Trichoderma fungus is cultured under the same conditions but without the glycosylamine.