US 12,227,780 B2
Isothiocyanate containing Brassicaceae products and method of preparation thereof
Mary Ann Augustin, Wheelers Hill (AU); and Netsanet Shiferaw Terefe, Werribee South (AU)
Assigned to COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, Acton (AU)
Appl. No. 16/651,974
Filed by Commonwealth Scientific and Industrial Research Organisation, Acton (AU)
PCT Filed Sep. 27, 2018, PCT No. PCT/AU2018/051063
§ 371(c)(1), (2) Date Mar. 27, 2020,
PCT Pub. No. WO2019/060963, PCT Pub. Date Apr. 4, 2019.
Claims priority of application No. 2017903944 (AU), filed on Sep. 28, 2017.
Prior Publication US 2020/0255872 A1, Aug. 13, 2020
Int. Cl. C12P 13/00 (2006.01); A23L 33/135 (2016.01); C12N 1/20 (2006.01)
CPC C12P 13/00 (2013.01) [A23L 33/135 (2016.08); C12N 1/20 (2013.01); A23V 2400/169 (2023.08); A23V 2400/321 (2023.08); C12Y 302/01147 (2013.01)] 15 Claims
 
1. A method of preparing an isothiocyanate containing product from Brassicaceae material comprising a glucosinolate comprising:
i) pre-treating the Brassicaceae material to improve the access of myrosinase to the glucosinolate; and
ii) fermenting the material obtained by step i) with lactic acid bacteria to form the isothiocyanate containing product,
wherein the lactic acid bacteria is selected from one or more of:
a) Leuconostoc mesenteroides BF1 deposited under V17/021729 on 25 Sep. 2017 at the National Measurement Institute Australia;
b) Leuconostoc mesenteroides BF2 deposited under V17/021730 on 25 Sep. 2017 at the National Measurement Institute Australia;
c) Lactobacillus plantarum B1 deposited under V17/021731 on 25 Sep. 2017 at the National Measurement Institute Australia;
d) Lactobacillus plantarum B2 deposited under V17/021732 on 25 Sep. 2017 at the National Measurement Institute Australia;
e) Lactobacillus plantarum B3 deposited under V17/021733 on 25 Sep. 2017 at the National Measurement Institute Australia;
f) Lactobacillus plantarum B4 deposited under V17/021734 on 25 Sep. 2017 at the National Measurement Institute Australia; and
e) Lactobacillus plantarum B5 deposited under V17/021735 on 25 Sep. 2017 at the National Measurement Institute Australia.