US 12,227,777 B2
Soluble alpha-Klotho proteins, protein fragments, and uses thereof
Moosa Mohammadi, Scarsdale, NY (US)
Assigned to NEW YORK UNIVERSITY, New York, NY (US)
Appl. No. 16/961,525
Filed by New York University, New York, NY (US)
PCT Filed Jan. 11, 2019, PCT No. PCT/US2019/013282
§ 371(c)(1), (2) Date Jul. 10, 2020,
PCT Pub. No. WO2019/140250, PCT Pub. Date Jul. 18, 2019.
Claims priority of provisional application 62/616,945, filed on Jan. 12, 2018.
Prior Publication US 2021/0054355 A1, Feb. 25, 2021
Int. Cl. A61K 38/00 (2006.01); C12N 9/24 (2006.01)
CPC C12N 9/2402 (2013.01) [A61K 38/00 (2013.01); C12Y 302/01031 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A modified soluble α-Klotho protein, wherein the modified soluble α-Klotho protein comprises a KL2 extracellular domain comprising a modification to eliminate binding affinity of the modified soluble α-Klotho protein for fibroblast growth factor receptor (FGFR) 1c, FGFR3c, and/or FGFR4, as compared to a wildtype soluble α-Klotho protein, wherein the wildtype soluble α-Klotho protein comprises the amino acid sequence of E34 to S981 of SEQ ID NO: 1, and wherein the modified soluble α-Klotho protein comprises the amino acid sequence of E34 to S932 of SEQ ID NO:2 or E34 to S959 of SEQ ID NO: 7.