US 12,227,773 B2
Recombinase polymerase amplification
Olaf Piepenburg, Cambridge (GB); Niall A. Armes, Essex (GB); and Matthew James David Parker, Hertfordshire (GB)
Assigned to ABBOTT DIAGNOSTICS SCARBOROUGH, INC., San Diego, CA (US)
Filed by ABBOTT DIAGNOSTICS SCARBOROUGH, INC., San Diego, CA (US)
Filed on May 23, 2022, as Appl. No. 17/750,758.
Application 17/750,758 is a continuation of application No. 16/155,133, filed on Oct. 9, 2018, granted, now 11,339,382, issued on May 24, 2022.
Application 16/155,133 is a continuation of application No. 14/135,227, filed on Dec. 19, 2013, granted, now 10,093,908, issued on Oct. 9, 2018.
Application 14/135,227 is a continuation of application No. 13/307,293, filed on Nov. 30, 2011, granted, now 8,637,253, issued on Jan. 28, 2014.
Application 13/307,293 is a continuation of application No. 11/800,318, filed on May 4, 2007, granted, now 8,071,308, issued on Dec. 6, 2011.
Claims priority of provisional application 60/798,060, filed on May 4, 2006.
Prior Publication US 2023/0242889 A1, Aug. 3, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/12 (2006.01); C12N 9/00 (2006.01); C12Q 1/6844 (2018.01)
CPC C12N 9/1241 (2013.01) [C12N 9/00 (2013.01); C12Q 1/6844 (2013.01); C12Y 207/07 (2013.01)] 7 Claims
 
1. A composition comprising:
a mutant UvsX protein comprising an amino acid substitution of a histidine in a Walker A box amino acid sequence; and
a crowding agent.