	US 12,227,761 B2
	Supportive nanofibrillar cellulose scaffold for expanding cells
Markus Nuopponen, Helsinki (FI); Lauri Paasonen, Järvenpää (FI); Susanna Narkilahti, Kangasala (FI); Tiina Joki, Lempäälä (FI); and Laura Ylä-Outinen, Tampere (FI)
Assigned to UPM-KYMMENE CORPORATION, Helsinki (FI)
Appl. No. 16/624,756
Filed by UPM-KYMMENE CORPORATION, Helsinki (FI)
PCT Filed Jun. 19, 2018, PCT No. PCT/FI2018/050478 § 371(c)(1), (2) Date Dec. 19, 2019, PCT Pub. No. WO2018/234634, PCT Pub. Date Dec. 27, 2018.
Claims priority of application No. 17177368 (EP), filed on Jun. 22, 2017.
Prior Publication US 2020/0115678 A1, Apr. 16, 2020
Int. Cl. C12N 5/0793 (2010.01); C08B 15/02 (2006.01)

CPC C12N 5/0619 (2013.01) [C08B 15/02 (2013.01); C12N 2501/73 (2013.01); C12N 2502/081 (2013.01); C12N 2513/00 (2013.01); C12N 2533/78 (2013.01)]

16 Claims

1. A composition configured for culture of expanding neuronal cells, said composition comprising:

0.05-0.35 wt % of mechanically disintegrated plant-derived anionic nanofibrillar cellulose having a degree of substitution of between 0.08 and 0.3 and a storage modulus between 5 and 20 Pa when dispersed to a concentration of 0.5 wt % in water, the nanofibrillar cellulose being in a form of hydrogel,

wherein said plant-derived anionic nanofibrillar cellulose comprises TEMPO oxidized nanofibrillar cellulose, and

wherein said plant-derived anionic nanofibrillar cellulose has a loss tangent greater than 1 when the range of storage modulus is between 1-20 Pa when dispersed to a concentration of 0.5 w % in water.