	US 12,227,759 B2
	Temperature-sensitive cell culture composition, method for using the same, method for forming the same, and use thereof
Chia-Jung Lu, Taichung (TW); Jing-En Huang, Hsinchu (TW); Liang-Cheng Su, Kaohsiung (TW); Hsin-Hsin Shen, Zhudong Township (TW); Yuchi Wang, New Taipei (TW); Ying-Hsueh Chao, Zhubei (TW); Li-Hsin Lin, Zhubei (TW); and Hsiu-Hua Huang, Hsinchu (TW)
Assigned to INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE, Hsinchu (TW)
Filed by Industrial Technology Research Institute, Hsinchu (TW)
Filed on Dec. 18, 2020, as Appl. No. 17/126,942.
Claims priority of provisional application 62/951,314, filed on Dec. 20, 2019.
Prior Publication US 2021/0269765 A1, Sep. 2, 2021
Int. Cl. C12N 5/00 (2006.01); A61K 9/00 (2006.01); A61K 35/12 (2015.01); A61K 47/38 (2006.01); A61K 47/42 (2017.01)

CPC C12N 5/0018 (2013.01) [A61K 9/0024 (2013.01); A61K 35/12 (2013.01); A61K 47/38 (2013.01); A61K 47/42 (2013.01); C12N 2523/00 (2013.01); C12N 2533/40 (2013.01); C12N 2533/54 (2013.01); C12N 2533/78 (2013.01); C12N 2539/00 (2013.01)]

16 Claims

1. A temperature-sensitive cell culture composition, comprising:

a hydrogel, wherein the hydrogel has a structure represented by Formula (I) or Formula (II) shown as follows:

A-B-BOX-B-A, Formula (I); or

B-A-B-(BOX-BAB)n-BOX-B-A-B, Formula (II),

wherein the polymer block A is a hydrophilic polyethylene glycol polymer, the polymer block B is a hydrophobic polyester polymer, BOX is a bifunctional group monomer of 2,2′-Bis(2-oxazoline) for coupling di-block of A-B or tri-block of B-A-B, and n is an integer greater than or equal to 0, and wherein an average molecular weight of the hydrogel is between 2000 and 6000,

wherein the polymer block A comprises polyethylene glycol (PEG) or methoxy-poly(ethylene glycol) (mPEG), and the polymer block B comprises poly(lactide-co-glycolide) (PLGA), poly(propionic-co-lactic (PPLA), poly(valeric-co-lactic) (PVLA) or poly(caproic-co-lactic) (PCLA);

a cellulose;

a gelatin; and

a collagen;

wherein based on 1 part by weight of the collagen, a content of the hydrogel is between 0.03 parts by weight and 60 parts by weight, a content of the cellulose is between 150 parts by weight and 360 parts by weight, and a content of the gelatin is between 1 part by weight and 12 parts by weight.