US 12,227,755 B2
Replication-defective arenavirus vectors
Daniel D. Pinschewer, Binningen (CH); Lukas Flatz, Schaan (LI); Andreas Bergthaler, Gmunden (AT); and Rolf Zinkernagel, Zumikon (CH)
Assigned to UNIVERSITÄT ZÜRICH, Zurich (CH)
Filed by UNIVERSITÄT ZÜRICH, Zürich (CH)
Filed on Jun. 22, 2022, as Appl. No. 17/846,817.
Application 16/861,758 is a division of application No. 15/928,003, filed on Mar. 21, 2018, granted, now 10,655,145, issued on May 19, 2020.
Application 17/846,817 is a continuation of application No. 16/861,758, filed on Apr. 29, 2020, granted, now 11,401,528.
Application 15/928,003 is a continuation of application No. 15/069,773, filed on Mar. 14, 2016, granted, now 9,944,952, issued on Apr. 17, 2018.
Application 15/069,773 is a continuation of application No. 14/061,025, filed on Oct. 23, 2013, granted, now 9,309,289, issued on Apr. 12, 2016.
Application 14/061,025 is a continuation of application No. 12/810,382, granted, now 8,592,205, issued on Nov. 26, 2013, previously published as PCT/EP2008/010994, filed on Dec. 22, 2008.
Claims priority of application No. 07025099 (EP), filed on Dec. 27, 2007.
Prior Publication US 2022/0315951 A1, Oct. 6, 2022
Int. Cl. C12N 15/86 (2006.01); A61K 35/76 (2015.01); A61K 39/12 (2006.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)
CPC C12N 15/86 (2013.01) [A61K 35/76 (2013.01); A61K 39/12 (2013.01); C07K 14/005 (2013.01); C12N 7/00 (2013.01); A61K 2039/525 (2013.01); A61K 2039/5256 (2013.01); C12N 2760/10032 (2013.01); C12N 2760/10034 (2013.01); C12N 2760/10043 (2013.01); C12N 2760/10061 (2013.01); C12N 2830/008 (2013.01); C12N 2840/203 (2013.01); C12N 2840/206 (2013.01); Y02A 50/30 (2018.01)] 7 Claims
 
1. A method of making a recombinant cell comprising transfecting a cell with plasmids encoding for intracellular expression of transacting factors (TF-plasmids), and plasmids encoding arenavirus vector genome segments (GS-plasmids),
wherein the GS-plasmid encodes a large segment of the arenavirus genome (L segment) or a small segment of the arenavirus genome (S segment),
wherein the GS-plasmid encoding the S segment comprises an arenavirus open reading frame wherein a sequence encoding for glycoprotein (GP) is removed and replaced by a foreign ribonucleic acid sequence,
wherein the foreign ribonucleic acid sequence encodes for (a) a viral antigen selected from a hepatitis B antigen, a respiratory syncytial virus antigen, a human immunodeficiency virus antigen, an influenza antigen, a hepatitis C virus antigen, a varicella zoster virus antigen, a herpes simplex virus antigen, or a cytomegalovirus antigen; (b) a bacterial antigen selected from a Haemophilus spp. antigen, a Pneumoccoccus spp. antigen, or a Mycobacterium tuberculosis antigen; (c) a parasite antigen selected from the group consisting of a plasmodia antigen, an amebia antigen, and a philaria antigen; or (d) a tumor antigen.