	US 12,227,753 B2
	CasY compositions and methods of use
Jennifer A. Doudna, Berkeley, CA (US); David Burstein, Berkeley, CA (US); Janice S. Chen, Berkeley, CA (US); Lucas B. Harrington, Berkeley, CA (US); David Paez-Espino, Walnut Creek, CA (US); and Jillian F. Banfield, Berkeley, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Appl. No. 16/755,534
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Oct. 31, 2018, PCT No. PCT/US2018/058522 § 371(c)(1), (2) Date Apr. 10, 2020, PCT Pub. No. WO2019/089804, PCT Pub. Date May 9, 2019.
Claims priority of provisional application 62/580,393, filed on Nov. 1, 2017.
Prior Publication US 2020/0255858 A1, Aug. 13, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01); C12N 15/85 (2006.01)

CPC C12N 15/85 (2013.01) [C12N 9/22 (2013.01); C12N 15/113 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)]

16 Claims

1. A composition comprising an engineered and/or non-naturally occurring complex comprising:

(a) a CasY polypeptide, or a nucleic acid encoding said CasY polypeptide, wherein the CasY polypeptide comprises an amino acid sequence having 97% or more amino acid sequence identity to the amino acid sequence set forth in any one of SEQ ID NOs: 1-2, 4, and 57;

(b) a CasY guide RNA, or a nucleic acid encoding said CasY guide RNA, wherein said CasY guide RNA comprises a guide sequence that is complementary to a target sequence of a target nucleic acid, and comprises a region that can bind to the CasY polypeptide; and