US 12,227,749 B2
Constructs for continuous monitoring of live cells
Paul Blainey, Cambridge, MA (US); Jacob Borrajo, Cambridge, MA (US); Mohamad Najia, Cambridge, MA (US); and Atray Dixit, Cambridge, MA (US)
Assigned to The Broad Institute, Inc., Cambridge, MA (US); and Massachusetts Institute of Technology, Cambridge, MA (US)
Filed by The Broad Institute, Inc., Cambridge, MA (US); and Massachusetts Institute of Technology, Cambridge, MA (US)
Filed on Jul. 14, 2023, as Appl. No. 18/352,347.
Application 18/352,347 is a continuation of application No. 16/335,512, granted, now 11,702,661, previously published as PCT/US2017/052822, filed on Sep. 21, 2017.
Claims priority of provisional application 62/397,867, filed on Sep. 21, 2016.
Prior Publication US 2024/0084311 A1, Mar. 14, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/62 (2006.01); C07H 21/04 (2006.01); C12Q 1/6897 (2018.01)
CPC C12N 15/62 (2013.01) [C07H 21/04 (2013.01); C12Q 1/6897 (2013.01); C12N 2310/20 (2017.05); C12N 2310/3519 (2013.01)] 31 Claims
 
1. A composition comprising:
a first nucleic acid sequence encoding a fusion protein, wherein the fusion protein comprises an export compartment protein and a construct RNA sequence capture domain, wherein the export compartment protein is a Gag protein and the construct RNA sequence capture domain comprises a Cas protein or an MS2 coat protein; and
a second nucleic acid sequence encoding a construct RNA sequence, wherein the construct RNA sequence comprises a barcode and a retrieval element,
wherein, when the first nucleic acid sequence and the second nucleic acid sequence are expressed the construct RNA sequence capture domain is capable of binding the retrieval element thereby associating the construct RNA sequence with the fusion protein.