	US 12,227,738 B2
	Method of single-stranded RNA purification
Špela Peršič Debenjak, Dobrovo (SI); Urh Černigoj, Ajdovscina (SI); Darko Dolenc, Vrhnika (SI); and Peter S. Gagnon, Las Vegas, NV (US)
Assigned to Sartorius BIA Separations d.o.o., Ajdovscina (SI)
Appl. No. 17/917,084
Filed by Sartorius BIA Separations d.o.o., Ajdovscina (SI)
PCT Filed Apr. 16, 2021, PCT No. PCT/EP2021/059903 § 371(c)(1), (2) Date Oct. 5, 2022, PCT Pub. No. WO2021/209595, PCT Pub. Date Oct. 21, 2021.
Claims priority of application No. 20170205 (EP), filed on Apr. 17, 2020.
Prior Publication US 2023/0151351 A1, May 18, 2023
Int. Cl. C12N 15/10 (2006.01)

CPC C12N 15/101 (2013.01)

20 Claims

1. A method of single-stranded RNA purification comprising the steps

applying a sample comprising single-stranded RNA to a solid phase having a surface bearing amino groups, wherein the amino groups comprise at least 50% primary amino groups, at a first pH value in a range sufficient to bind the single-stranded RNA,

eluting the single stranded RNA from the surface of the solid phase by exposing the surface of the solid phase to an ascending pH gradient having a range of pH values that are higher than the first pH value, wherein the single-stranded RNA is collected as a purified fraction.