US 12,227,734 B2
Microscale bioprocessing system and method for protein manufacturing from human blood
Govind Rao, Ellicott City, MD (US); Yordan Kostov, Columbia, MD (US); Leah Tolosa Croucher, Columbia, MD (US); Kevin Tran, Marriotsville, MD (US); Monohar Pilli, Gwynn Oak, MD (US); Michael Tolosa, Columbia, MD (US); Chandrasekhar Gurramkonda, Halethorpe, MD (US); and David Burgenson, Daly City, CA (US)
Assigned to University of Maryland, Baltimore County, Baltimore, MD (US)
Filed by University of Maryland, Baltimore County, Baltimore, MD (US)
Filed on Jun. 10, 2022, as Appl. No. 17/837,772.
Application 17/837,772 is a continuation of application No. 15/329,555, abandoned, previously published as PCT/US2015/043314, filed on Jul. 31, 2015.
Claims priority of provisional application 62/031,484, filed on Jul. 31, 2014.
Prior Publication US 2022/0298469 A1, Sep. 22, 2022
Int. Cl. C07K 9/00 (2006.01); A61K 35/14 (2015.01); A61M 1/36 (2006.01); B01D 15/08 (2006.01); B01D 15/32 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); C07K 1/36 (2006.01); C12M 1/00 (2006.01); C12M 1/40 (2006.01)
CPC C12M 47/12 (2013.01) [A61K 35/14 (2013.01); A61M 1/3621 (2013.01); B01D 15/08 (2013.01); B01D 15/327 (2013.01); B01D 15/361 (2013.01); B01D 15/3847 (2013.01); C07K 1/36 (2013.01); C12M 21/18 (2013.01); C12M 29/04 (2013.01)] 19 Claims
 
1. A system for expressing and purifying a desired protein from blood, comprising:
a whole blood separator adapted for receiving whole blood and outputting (i) at least one blood fraction comprising at least one of erythrocytes, reticulocytes and lymphocytes and (ii) a plasma and unused portion fraction;
a blood cell lysing module adapted for receiving and lysing the at least one blood fraction from the whole blood separator and outputting a lysate;
a bioreactor, adapted for producing the desired protein utilizing cell-free protein expression, for receiving the lysate from the blood cell lysing module and producing the desired protein from the lysate via protein expression utilizing cDNA or mRNA for the desired protein; and
a purification module for receiving the desired protein from the bioreactor and purifying the desired protein.