US 12,227,732 B2
Systems for cell culture scaling
Christopher Brau, Grand Island, NY (US); Nephi Jones, Newton, UT (US); and Mark Smith, Nibley, UT (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Oct. 31, 2022, as Appl. No. 18/051,126.
Application 18/051,126 is a continuation of application No. 16/238,591, filed on Jan. 3, 2019, granted, now 11,584,910.
Claims priority of provisional application 62/712,343, filed on Jul. 31, 2018.
Claims priority of provisional application 62/670,934, filed on May 14, 2018.
Claims priority of provisional application 62/618,215, filed on Jan. 17, 2018.
Prior Publication US 2023/0091993 A1, Mar. 23, 2023
Int. Cl. B01F 23/00 (2022.01); B01F 23/233 (2022.01); B01F 27/054 (2022.01); B01F 27/114 (2022.01); B01F 27/213 (2022.01); B01F 27/92 (2022.01); B01F 35/32 (2022.01); B01F 35/41 (2022.01); B01F 35/43 (2022.01); B01F 35/513 (2022.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/36 (2006.01); C12M 1/42 (2006.01); C12N 5/071 (2010.01); B01F 27/90 (2022.01); B01F 35/30 (2022.01); B01F 101/44 (2022.01)
CPC C12M 35/04 (2013.01) [B01F 23/233 (2022.01); B01F 27/054 (2022.01); B01F 27/114 (2022.01); B01F 27/213 (2022.01); B01F 27/92 (2022.01); B01F 35/3204 (2022.01); B01F 35/4121 (2022.01); B01F 35/43 (2022.01); B01F 35/513 (2022.01); C12M 23/50 (2013.01); C12M 23/58 (2013.01); C12M 27/02 (2013.01); C12M 27/06 (2013.01); C12M 41/48 (2013.01); C12N 5/0602 (2013.01); B01F 23/23362 (2022.01); B01F 27/90 (2022.01); B01F 35/3214 (2022.01); B01F 2035/352 (2022.01); B01F 2101/44 (2022.01); C12N 2527/00 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A scalable bioreactor system for transitioning from testing to commercial production, comprising:
a first bioreactor, comprising:
a first bioprocessing container having a first end, a second end, a sidewall, and a first sparger affixed to the first end of the first bioprocessing container and having a first number of pores, each pore of the first number of pores having a first diameter;
a first configurable mixing assembly suspended between the first and second ends of the first bioprocessing container; and
a first impeller having a first diameter, the first impeller attached to the first configurable mixing assembly in a first position, wherein the first diameter and the first position are selected to achieve a set of operational parameters;
a second bioreactor, comprising
a second bioprocessing container having a first end, a second end, a sidewall, and a second sparger affixed to the first end of the second bioprocessing container and having a second number of pores, each pore of the second number of pores having a second diameter, wherein the second bioprocessing container is not the same volume as the first bioprocessing container;
a second configurable mixing assembly suspended between the first and second ends of the second bioprocessing container; and
a second impeller having a second diameter that is not the same as the first diameter, the second impeller attached to the second configurable mixing assembly in a second position, wherein the second diameter and the second position are selected to match the set of operational parameters and the operational parameters include power per volume and impeller tip speed.