US 12,227,730 B2
System and method for aseptic sampling and fluid addition
Mark Robert Timmins, Chelmsford, MA (US); Matthew Sherman, West Newton, MA (US); Yasser S. Ali, Westborough, MA (US); Marc Picardo, Ashland, MA (US); and Keith Benoit, Grafton, MA (US)
Assigned to GLOBAL LIFE SCIENCES SOLUTIONS USA LLC, Marlborough, MA (US)
Filed by GLOBAL LIFE SCIENCES SOLUTIONS USA LLC, Marlborough, MA (US)
Filed on Nov. 18, 2020, as Appl. No. 16/951,280.
Prior Publication US 2022/0154127 A1, May 19, 2022
Int. Cl. C12M 1/32 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); G01N 1/28 (2006.01)
CPC C12M 33/06 (2013.01) [C12M 27/20 (2013.01); C12M 29/04 (2013.01); C12M 33/04 (2013.01); G01N 1/28 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A sampling system, comprising:
a sample source having a volume of fluid;
a first graduated sampling chamber;
a first flow line fluidly connecting the sample source with the first graduated sampling chamber;
a first check valve operatively coupled to the first flow line intermediate of the first graduated sampling chamber and the sample source, the first check valve having a direct connection with the first graduated sampling chamber via the first flow line without any additional components therebetween, wherein the first check valve is configured to permit only unidirectional flow of the fluid from the sample source to the first sampling chamber via the first flow line, and wherein the flow of the fluid from the sample source to the first graduated sampling chamber flows directly from the first check valve into the first graduated sampling chamber without passage through any additional components operatively coupled to the first flow line between the sample source and the first graduated sampling chamber;
a first pump device configured for fluid connection with the first graduated sampling chamber;
a sterile air filter intermediate the first pump device and the first graduated sampling chamber;
a media source;
a second graduated sampling chamber configured for fluid connection to the media source via an inlet port and for fluid connection to the sample source via an outlet port; and
a second pump device configured for fluid connection with the second graduated sampling chamber;
wherein the first pump device is selectively actuatable to draw the volume of fluid from the sample source into the first graduated sampling chamber without the volume of fluid contacting the first pump device,
wherein the second pump device is selectively actuatable to draw a volume of fluid from the media source into the second graduated sampling chamber, and to push the volume of fluid from the second graduated sampling chamber into the sample source.