US 12,227,726 B2
Multigel tumor-on-a-chip system
Mu-Ping Nieh, Mansfield, CT (US); Armin Tahmasbi Rad, Manchester, CT (US); Reza Amin, Farmington, CT (US); and Leila Daneshmandi, Manchester, CT (US)
Assigned to University of Connecticut, Farmington, CT (US)
Filed by University of Connecticut, Farmington, CT (US)
Filed on Aug. 3, 2023, as Appl. No. 18/364,999.
Application 18/364,999 is a continuation of application No. 16/899,676, filed on Jun. 12, 2020, granted, now 11,760,966.
Claims priority of provisional application 62/861,456, filed on Jun. 14, 2019.
Prior Publication US 2024/0067911 A1, Feb. 29, 2024
Int. Cl. C12M 3/06 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12N 5/00 (2006.01); G01N 33/50 (2006.01)
CPC C12M 23/16 (2013.01) [C12M 25/16 (2013.01); C12M 41/46 (2013.01); C12N 5/0012 (2013.01); G01N 33/5008 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A biomimetic microfluidic device for culturing cells, the device comprising:
a plurality of separate growing chambers, each growing chamber suited for providing a microenvironment for culturing a population of tumor cells;
a free flow space connected to a top portion of each growing chamber, wherein the free flow space is configured to mimic an area of a blood vessel positioned directly adjacent to a tumor, pumping nutrients to each population of tumor cells; and,
one or more flow chambers comprising an inlet for receiving cell culture media and an outlet for releasing cell culture media, wherein the one or more flow chambers are directly connected to the free flow space and are in fluid communication with each growing chamber through the free flow space.