	US 12,227,723 B2
	Variable diameter bioreactors
Michael Mietzner, Fremont, NH (US); Rajesh Beri, Westford, MA (US); and Edward Gunderson, Nottingham, NH (US)
Assigned to Lonza Ltd, Visp (CH)
Filed by Lonza Ltd, Visp (CH)
Filed on Feb. 1, 2023, as Appl. No. 18/162,774.
Application 18/162,774 is a division of application No. 16/406,067, filed on May 8, 2019, granted, now 11,597,900.
Application 16/406,067 is a division of application No. 15/629,886, filed on Jun. 22, 2017, granted, now 10,370,629, issued on Aug. 6, 2019.
Claims priority of provisional application 62/354,216, filed on Jun. 24, 2016.
Prior Publication US 2023/0183623 A1, Jun. 15, 2023
Int. Cl. C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/36 (2006.01); C12P 21/00 (2006.01)

CPC C12M 23/02 (2013.01) [C12M 23/14 (2013.01); C12M 23/28 (2013.01); C12M 27/02 (2013.01); C12M 41/48 (2013.01); C12M 43/00 (2013.01); C12P 21/00 (2013.01); C12P 21/005 (2013.01)]

15 Claims

1. A bioproduction facility, comprising:

(a) an initial inoculum growth reactor, and

(b) a variable diameter bioreactor, wherein the variable diameter bioreactor is in fluid communication with the initial inoculum growth reactor,

wherein the variable diameter bioreactor comprises a conical section that defines an interior conical space, wherein the interior conical space can be partially or fully filled with a cell culture that is grown in the variable diameter bioreactor,

and wherein the variable diameter bioreactor functions as a seed stage reactor train.