	US 12,227,695 B2
	Glycosyl hydrolase enzymes in high temperature industrial processes
Robert Muko Tjon-Joe-Pin, Spring, TX (US); Moreland David Gibbs, Orakei (NZ); and Valentino Setoa Junior Te'o, Brisbane (AU)
Assigned to Advanced Enzyme Systems, LLC, Spring, TX (US)
Filed by Advanced Enzyme Systems, LLC, Spring, TX (US)
Filed on Jul. 22, 2022, as Appl. No. 17/871,025.
Application 17/871,025 is a division of application No. 16/212,470, filed on Dec. 6, 2018, granted, now 11,401,457.
Application 16/212,470 is a division of application No. 14/553,701, filed on Nov. 25, 2014, granted, now 10,227,523, issued on Mar. 12, 2019.
Claims priority of provisional application 61/909,012, filed on Nov. 26, 2013.
Prior Publication US 2023/0183559 A1, Jun. 15, 2023
Int. Cl. C09K 8/68 (2006.01); C12N 9/24 (2006.01)

CPC C09K 8/68 (2013.01) [C12N 9/2491 (2013.01); C12N 9/2494 (2013.01); C09K 2208/24 (2013.01)]

11 Claims

1. A mutated recombinant hyperthermophilic Dictyoglomus beta-mannanase (DtManA) enzyme comprising a Carbohydrate Binding Module (CBM) domain having an 88% amino acid sequence identity with a native DtManA CBM of SEQ ID NO: 21 and a catalytic domain having an 97% amino acid sequence identity with a native DtManA catalytic domain of SEQ ID NO: 3, wherein the CBM domain is mutated to reduce or abolish mannan binding by amino acid substitution at a key residue for mannan binding selected from the group consisting of one or more of: lysine residue at 46 on SEQ ID NO: 21; tryptophan residue at 109 on SEQ ID NO: 21; and tryptophan residue at 111 on SEQ ID NO: 21.