US 12,227,558 B2
Modulators of notch signaling and methods of use thereof
Thomas J. Magliery, Columbus, OH (US); Mikhail M. Dikov, Dublin, OH (US); David Carbone, Hilliard, OH (US); Nicholas Long, Columbus, OH (US); Brandon Sullivan, Gahanna, OH (US); and Elena Tchekneva, Dublin, OH (US)
Assigned to Ohio State Innovation Foundation, Columbus, OH (US)
Filed by Ohio State Innovation Foundation, Columbus, OH (US)
Filed on Feb. 10, 2023, as Appl. No. 18/167,121.
Application 18/167,121 is a continuation of application No. 16/496,163, granted, now 11,613,567, previously published as PCT/US2018/024343, filed on Mar. 26, 2018.
Claims priority of provisional application 62/476,616, filed on Mar. 24, 2017.
Prior Publication US 2024/0018213 A1, Jan. 18, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/00 (2006.01); A61P 35/00 (2006.01); A61P 37/06 (2006.01); C07K 14/705 (2006.01)
CPC C07K 14/70596 (2013.01) [A61P 35/00 (2018.01); A61P 37/06 (2018.01); A61K 38/00 (2013.01)] 19 Claims
 
1. A Notch-modulating peptide comprising at least four monomeric units connected by peptide linkers in a single genetically-encoded peptide, the monomeric units comprising:
(a) a mammalian DSL domain; and
(b) two or three mammalian EGF domains, wherein the two or three mammalian EGF domains independently comprise a sequence selected from SEQ ID NO:154, SEQ ID NO: 155, SEQ ID NO:156, or a sequence that is at least 80% identical to SEQ ID NO: 154, SEQ ID NO:155, or SEQ ID NO:156;
wherein the peptide linkers comprise the amino acid sequence GGGGS (SEQ ID NO: 132), GSSGSSG (SEQ ID NO:133), or a sequence that is at least 60% identical to SEQ ID NO: 132 or SEQ ID NO: 133; and
wherein the mammalian DSL domain comprises SEQ ID NO:153, or a sequence that is at least 80% identical to SEQ ID NO:153.