| CPC C07H 1/06 (2013.01) [C07H 19/048 (2013.01)] | 10 Claims |
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1. A method for preparing nicotinamide mononucleotide by using nicotinamide as a raw material, wherein the method comprises steps of:
1) allowing nicotinamide and tetraacetyl ribose to react in acetonitrile, dichloromethane, 1,2-dichloroethane or liquid sulfur dioxide as a solvent as catalyzed by trimethylsilyl trifluoromethanesulfonate or tin tetrachloride at a temperature of 20-40° C. to obtain a first reaction mixture;
2) adding sodium bicarbonate, sodium carbonate or sodium hydroxide into the first reaction mixture to adjust a pH value thereof to 3-5 to obtain a second reaction mixture;
3) adding a sodium methoxide solution into the second reaction mixture to react at a temperature of −15° C. to 5° C. to obtain a third reaction mixture;
4) adding hydrochloric acid into the third reaction mixture to adjust a pH value thereof to 3-5 to obtain a fourth reaction mixture;
5) performing microfiltration and nanofiltration on the fourth reaction mixture in sequence by using a membrane concentrator to obtain a nicotinamide ribose solution; and
6) allowing the nicotinamide ribose solution obtained in the step 5) to react as catalyzed by nicotinamide ribokinase at a temperature of 35-39° C. in the presence of Mg ions, ATP and a buffer, wherein a pH value of the reaction mixture is controlled at 7.5-8.0 during the reaction, and nicotinamide mononucleotide is obtained after the reaction is complete.
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