US 12,227,529 B2
Method of preparing silanols with selective cytochrome P450 variants and related compounds and compositions
John Roberts, Midland, MI (US); Dimitris Elias Katsoulis, Midland, MI (US); Susanne Baehr, Pasadena, CA (US); Sabine Brinkmann-Chen, Pasadena, CA (US); S. B. Jennifer Kan, Pasadena, CA (US); and Frances H. Arnold, Pasadena, CA (US)
Assigned to DOW SILICONES CORPORATION, Midland, MI (US); and CALIFORNIA INSTITUTE OF TECHNOLOGY, Pasadena, CA (US)
Appl. No. 17/800,211
Filed by DOW SILICONES CORPORATION, Midland, MI (US); and CALIFORNIA INSTITUTE OF TECHNOLOGY, Pasadena, CA (US)
PCT Filed Feb. 19, 2021, PCT No. PCT/US2021/018749
§ 371(c)(1), (2) Date Aug. 16, 2022,
PCT Pub. No. WO2021/168228, PCT Pub. Date Aug. 26, 2021.
Claims priority of provisional application 62/979,960, filed on Feb. 21, 2020.
Prior Publication US 2023/0102728 A1, Mar. 30, 2023
Int. Cl. C07F 7/08 (2006.01)
CPC C07F 7/0836 (2013.01) 20 Claims
 
1. A method of preparing a silanol-functional organosilicon compound, said method comprising:
combining a cytochrome P450 variant that facilitates the oxidization of a silyl hydride group to a silanol group in the presence of oxygen and an organosilicon compound having at least one silicon-bonded hydrogen atom to give a reaction mixture; and
exposing the reaction mixture to oxygen to oxidize the organosilicon compound, thereby preparing the silanol-functional organosilicon compound.