US 12,226,784 B2
Magnetic bead purification system
Jinxin Zhu, Jiangsu (CN); Ruina He, Jiangsu (CN); Hong Qian, Jiangsu (CN); Tao Bai, Jiangsu (CN); Deming Li, Jiangsu (CN); Cheng Zheng, Jiangsu (CN); and Guodong Chen, Jiangsu (CN)
Assigned to Nanjing GenScript Biotech Co., Ltd., Jiangsu (CN)
Appl. No. 17/051,119
Filed by Nanjing GenScript Biotech Co., Ltd., Jiangsu (CN)
PCT Filed Apr. 26, 2019, PCT No. PCT/CN2019/084590
§ 371(c)(1), (2) Date Oct. 27, 2020,
PCT Pub. No. WO2019/206284, PCT Pub. Date Oct. 31, 2019.
Claims priority of application No. 201810403510.1 (CN), filed on Apr. 28, 2018.
Prior Publication US 2021/0299677 A1, Sep. 30, 2021
Int. Cl. B03C 1/28 (2006.01); C07K 1/14 (2006.01); C12M 1/00 (2006.01); C12M 1/32 (2006.01); C12M 1/42 (2006.01); C12N 15/10 (2006.01)
CPC B03C 1/288 (2013.01) [C07K 1/14 (2013.01); C12M 1/42 (2013.01); C12M 33/06 (2013.01); C12M 47/04 (2013.01); C12M 47/12 (2013.01); C12N 15/1013 (2013.01); B03C 2201/18 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A magnetic bead purification system, comprising:
a housing;
a purification station system movable between a purification treatment position inside the housing and a loading position outside the housing, the purification station system being adapted to load a container filled with a mixed liquid of incubated crude proteins and magnetic beads;
a liquid path treatment system provided inside the housing, the liquid path treatment system being connectable to a reagent barrel and a waste liquid barrel;
a sample addition needle group connected to the liquid path treatment system so as to receive a reagent from the liquid path treatment system or to discharge a waste liquid to the liquid path treatment system, the sample addition needle group being movable within the housing;
wherein each sample addition needle of the sample addition needle group comprises a waste discharging needle hole configured to discharge waste liquid from said container at the purification treatment position and a sample addition needle hole configured to add reagents to said container at the purification treatment position;
wherein the sample addition needle holes are further configured for spray rinsing of a container wall of said container and bubble blowing in said container at the purification treatment position; and
a purification magnetic separation system comprising a magnetic element, wherein the magnetic element is a permanent magnet, and wherein the purification magnetic separation system is controllable to apply a lateral magnetic force to said container at the purification treatment position inside the housing or stop the application of the lateral magnetic force by moving the magnetic element toward and away from the purification treatment position, and the magnetic element applies the lateral magnetic force to attract the magnetic beads onto the container wall of said container; and
wherein the purification station system comprises a magnetic attraction block so as to move from the loading position to the purification treatment position by the lateral magnetic force of the magnetic element of the purification magnetic separation system.