US 12,226,773 B2
Method and device for encapsulating cell in liquid droplet for single-cell analysis
Woong-Yang Park, Seoul (KR); Hui-Sung Moon, Gyeonggi-do (KR); Shin-Hyun Kim, Daejeon (KR); and Kwanghwi Je, Daejeon (KR)
Assigned to SAMSUNG LIFE PUBLIC WELFARE FOUNDATION, Seoul (KR)
Filed by Samsung Life Public Welfare Foundation, Seoul (KR)
Filed on Oct. 10, 2022, as Appl. No. 17/962,752.
Application 17/962,752 is a division of application No. 16/686,613, filed on Nov. 18, 2019, abandoned.
Application 16/686,613 is a continuation in part of application No. PCT/KR2018/005646, filed on May 17, 2018.
Claims priority of application No. 10-2017-0061053 (KR), filed on May 17, 2017.
Prior Publication US 2023/0030813 A1, Feb. 2, 2023
Int. Cl. C12Q 1/6869 (2018.01); B01L 3/00 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502776 (2013.01); B01L 3/502784 (2013.01); C12Q 1/6869 (2013.01); B01L 2200/0636 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0867 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A method of forming droplets for single-cell analysis, the method comprising:
providing cells;
providing one or more distinctly barcoded RNA capture beads;
providing one or more oil phase;
encapsulating in one droplet a single cell of the cells and a single bead of the one or more distinctly barcoded RNA capture beads; and
mixing the cells and beads encapsulated in the droplet,
wherein the providing of the cells or the providing of the one or more distinctly barcoded RNA capture beads is provided through inertial ordering before the single cell or the single bead are encapsulated in the one droplet, and
wherein the inertial ordering occurs through a spiral channel of a microfluidic device to which the cells or the one or more distinctly barcoded RNA capture beads are provided,
wherein the oil phase in the providing one or more oil phase step has a flow rate in a range of 4.0 mL/h to 8.0 mL/h, and
wherein the cells in the providing cells step and beads in the providing one or more distinctly barcoded RNA capture beads step have a flow rate in a range of 8.0 mL/h to 14 mL/h 6.0 mL/h.