US 12,226,770 B2
Methods and apparatuses for sorting target particles
Ivan K. Dimov, Mountain View, CA (US); Nathaniel Fernhoff, Menlo Park, CA (US); and Lagnajeet Pradhan, Union City, CA (US)
Assigned to Orca Biosystems, Inc., Menlo Park, CA (US)
Filed by Orca Biosystems, Inc., Menlo Park, CA (US)
Filed on Aug. 4, 2023, as Appl. No. 18/365,740.
Application 18/365,740 is a continuation of application No. 17/942,409, filed on Sep. 12, 2022, granted, now 11,759,779.
Application 17/942,409 is a continuation of application No. 16/903,103, filed on Jun. 16, 2020, granted, now 11,471,885, issued on Oct. 18, 2022.
Application 16/903,103 is a continuation of application No. 16/425,856, filed on May 29, 2019, granted, now 10,722,885, issued on Jul. 28, 2020.
Application 16/425,856 is a continuation of application No. 16/044,166, filed on Jul. 24, 2018, granted, now 10,350,595, issued on Jul. 16, 2019.
Application 16/044,166 is a continuation of application No. PCT/US2017/061414, filed on Nov. 13, 2017.
Claims priority of provisional application 62/421,979, filed on Nov. 14, 2016.
Prior Publication US 2024/0075471 A1, Mar. 7, 2024
Int. Cl. B01L 3/00 (2006.01); B01L 9/00 (2006.01); G01N 15/1434 (2024.01); G01N 21/64 (2006.01); G02B 21/16 (2006.01); H01S 5/183 (2006.01); H01S 5/42 (2006.01); G01N 15/149 (2024.01)
CPC B01L 3/502715 (2013.01) [B01L 3/50853 (2013.01); B01L 3/5088 (2013.01); B01L 9/523 (2013.01); G01N 15/1436 (2013.01); G01N 21/6402 (2013.01); G01N 21/6458 (2013.01); G02B 21/16 (2013.01); H01S 5/18388 (2013.01); H01S 5/423 (2013.01); B01L 2200/0605 (2013.01); B01L 2300/0829 (2013.01); G01N 15/149 (2024.01)] 19 Claims
OG exemplary drawing
 
1. A method of loading a mixture into a microchannel of substrate comprising a plurality of microchannels, the method comprising:
adding a first mixture to microchannels of a substrate, wherein the first mixture comprises a transparent solution and a plurality of particles; and
adding a second mixture to microchannels of a substrate, wherein the second mixture comprises a sample component and an aqueous solution.