(a) obtaining a plurality of supports wherein each support has a plurality of single-stranded oligonucleotide primers immobilized to the support and has a template nucleic acid immobilized to the support, wherein the template nucleic acid comprises a sequence of contiguous nucleotides at an end of the template nucleic acid strand that is immobilized to the support that is the same as a sequence of the oligonucleotide primers;

(b) subjecting the template nucleic acids immobilized to the plurality of supports to a first isothermal nucleic acid amplification in the presence of (i) a first primer attached to a linker moiety in solution and (ii) a capture moiety that attaches to the linker moiety, wherein the first primer comprises a sequence of nucleotides that is complementary to a primer-binding sequence of the immobilized template nucleic acid strand at an end of the strand opposite to the end immobilized to the support, the capture moiety attached to a bead or particle; wherein, following step (b):

(i) each support of the plurality of supports of step (b) has a different double-stranded template nucleic acid attached thereto,

(ii) each double-stranded template nucleic acid comprises the immobilized template nucleic acid strand that is directly attached to the support and a strand that is hybridized to the immobilized template nucleic acid strand, and

(iii) each double-stranded template nucleic acid comprises the linker moiety on the strand that is hybridized to the immobilized template nucleic acid strand;

(c) forming a plurality of captured supports by contacting the plurality of supports of step (b) with the capture moiety that binds to the linker moiety;

(d) collecting the captured supports;

(e) separating the supports having template nucleic acids immobilized thereto from the capture moiety; and

(f) subjecting the immobilized nucleic acids attached to the plurality of supports to a second isothermal nucleic acid amplification in the presence of the first primer, attached to the linker moiety or not attached to the linker moiety, in solution and in the absence of the capture moiety that attaches to the linker moiety, wherein the second isothermal nucleic acid amplification is a recombinase-polymerase amplification, thereby generating substantially monoclonal populations of template nucleic acids attached to the supports.