US 12,226,529 B2
Stable nanolipoprotein particles and related compositions methods and systems
Craig D. Blanchette, Concord, CA (US); Nicholas Fischer, Livermore, CA (US); Sean Fitzpatrick Gilmore, Oakland, CA (US); Amy Rasley, Livermore, CA (US); and Paul Henderson, Dublin, CA (US)
Assigned to LAWRENCE LIVERMORE NATIONAL SECURITY, LLC, Livermore, CA (US)
Appl. No. 15/755,018
Filed by LAWRENCE LIVERMORE NATIONAL SECURITY, LLC, Livermore, CA (US); and THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
PCT Filed Aug. 25, 2016, PCT No. PCT/US2016/048632
§ 371(c)(1), (2) Date Feb. 23, 2018,
PCT Pub. No. WO2017/035326, PCT Pub. Date Mar. 2, 2017.
Claims priority of provisional application 62/209,784, filed on Aug. 25, 2015.
Prior Publication US 2019/0142752 A1, May 16, 2019
Int. Cl. A61K 9/127 (2006.01); A61K 47/69 (2017.01); A61K 49/00 (2006.01); C07F 9/10 (2006.01); C07K 14/705 (2006.01); C07K 14/775 (2006.01); B82Y 5/00 (2011.01)
CPC A61K 9/1273 (2013.01) [A61K 9/1278 (2013.01); A61K 47/6915 (2017.08); A61K 49/0086 (2013.01); C07F 9/106 (2013.01); C07K 14/705 (2013.01); C07K 14/775 (2013.01); B82Y 5/00 (2013.01)] 36 Claims
 
1. A nanolipoprotein particle comprising:
a membrane forming lipid, a polymerized lipid and a scaffold protein,
the membrane forming lipid and the polymerized lipid arranged in a membrane forming lipid discoidal bilayer stabilized by the scaffold protein and by the polymerized lipid,
wherein the polymerized lipid is formed by crosslinking polymerizable lipids within the membrane forming lipid discoidal bilayer in a molar concentration of about 5 to about 40 mol %.