US 12,226,477 B2
Methods for preparing complex for enhancing immune response
Haixiang Lin, Beijing (CN); Fang Liu, Beijing (CN); and Li Zha, Beijing (CN)
Assigned to BEIJING YISHENG BIOTECHNOLOGY CO., LTD., Beijing (CN)
Appl. No. 17/256,980
Filed by XINFU (BEIJING) MEDICAL TECHNOLOGY CO., LTD., Beijing (CN)
PCT Filed Jun. 28, 2019, PCT No. PCT/CN2019/093607
§ 371(c)(1), (2) Date Mar. 31, 2021,
PCT Pub. No. WO2020/001596, PCT Pub. Date Jan. 2, 2020.
Claims priority of application No. 201810698033.6 (CN), filed on Jun. 29, 2018; and application No. 201810700708.6 (CN), filed on Jun. 29, 2018.
Prior Publication US 2023/0210893 A1, Jul. 6, 2023
Prior Publication US 2024/0316189 A9, Sep. 26, 2024
Int. Cl. A61K 39/39 (2006.01); A61K 31/787 (2006.01); A61K 45/06 (2006.01); A61K 47/02 (2006.01); A61K 47/26 (2006.01); A61K 47/36 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/39 (2013.01) [A61K 31/787 (2013.01); A61K 45/06 (2013.01); A61K 47/02 (2013.01); A61K 47/26 (2013.01); A61K 47/36 (2013.01); A61K 2039/55555 (2013.01); A61K 2039/55561 (2013.01)] 18 Claims
 
1. A method for preparing a complex for potentiating an immune response, comprising:
heating a polyinosinic-polycytidylic acid at 80° C.˜99° C. for 70 min˜120 min, and then
contacting a polyinosinic-polycytidylic acid, at least a cationic stabilizer, and a soluble calcium salt in a liquid reaction system;
the cationic stabilizer is a water-soluble non-antibiotic amino compound having a molecular weight of less than or equal to 5 kDa, or a graft formed by the water-soluble non-antibiotic amino compound and one or more of methoxypolyethylene glycol, polyethylene glycol, polyethylenimine, folic acid and galactose.