	US 12,226,472 B2
	Foot-and-mouth disease virus-like particle antigen, and vaccine composition, preparation method, and application thereof
Kegong Tian, Henan (CN); Yan Xiao, Henan (CN); Wenqiang Pang, Henan (CN); Jinzhong Sun, Henan (CN); and Xuke Zhang, Henan (CN)
Assigned to PULIKE BIOLOGICAL ENGINEERING, INC., Henan (CN)
Appl. No. 17/421,541
Filed by PULIKE BIOLOGICAL ENGINEERING, INC., Henan (CN)
PCT Filed Jan. 15, 2019, PCT No. PCT/CN2019/071813 § 371(c)(1), (2) Date Jul. 8, 2021, PCT Pub. No. WO2020/147015, PCT Pub. Date Jul. 23, 2020.
Prior Publication US 2022/0096620 A1, Mar. 31, 2022
Int. Cl. A61K 39/135 (2006.01); A61K 39/00 (2006.01); A61P 31/14 (2006.01); C07K 14/005 (2006.01)

CPC A61K 39/135 (2013.01) [A61P 31/14 (2018.01); C07K 14/005 (2013.01); A61K 2039/5258 (2013.01); A61K 2039/55577 (2013.01)]

11 Claims

1. A type O foot-and-mouth disease virus-like particle vaccine, wherein the type O foot-and-mouth disease virus-like particle vaccine comprises a type O CATHAY topotype foot-and-mouth disease virus-like particle antigen and a pharmaceutically acceptable carrier that includes an adjuvant:

wherein the type O foot-and-mouth disease virus-like particle antigen is a type O CATHAY topotype foot-and-mouth disease virus-like particle antigen, and the type O CATHAY topotype foot-and-mouth disease virus-like particle antigen is assembled by VP0, VP3 and VP1 antigen proteins of a type O CATHAY topotype foot-and-mouth disease virus (FMDV);

wherein the VP0 antigen protein of the type O CATHAY topotype FMDV is encoded by SEQ ID NO: 1 or a degenerate sequence thereof; the VP3 antigen protein of the type O CATHAY topotype FMDV is encoded by SEQ ID NO: 2 or a degenerate sequence thereof; and the VP1 antigen protein of the type O CATHAY topotype FMDV is encoded by SEQ ID NO: 3 or a degenerate sequence thereof;

wherein the type O foot-and-mouth disease virus-like particle vaccine further comprises an immunogenic amount of type O Southeast Asian (SEA) topotype foot-and-mouth disease virus-like particle antigen, and the type O SEA topotype foot-and-mouth disease virus-like particle antigen is assembled by VP4, VP2, VP3 and VP1 antigen proteins of type O SEA topotype FMDV; and

wherein the VP4 antigen protein of type O SEA topotype FMDV is encoded by SEQ ID NO: 4 or a degenerate sequence thereof; the VP2 antigen protein of type O SEA topotype FMDV is encoded by SEQ ID NO: 5 or a degenerate sequence thereof; the VP3 antigen protein of type O SEA topotype FMDV is encoded by SEQ ID NO: 6 or a degenerate sequence thereof; and the VP1 antigen protein of type O SEA topotype FMDV is encoded by SEQ ID NO: 7 or a degenerate sequence thereof.