US 12,226,469 B2
Proteins and nucleic acids useful in vaccines targeting Klebsiella pneumoniae
Niels Iversen Møller, København K (DK); and Andreas Holm Mattsson, København K (DK)
Assigned to Evaxion Biotech A/S, Hørsholm (DK)
Filed by Evaxion Biotech ApS, København K (DK)
Filed on Nov. 4, 2020, as Appl. No. 17/088,683.
Application 17/088,683 is a continuation of application No. 16/594,987, filed on Oct. 7, 2019, granted, now 10,849,968.
Application 16/594,987 is a continuation of application No. 15/542,580, granted, now 10,434,162, issued on Oct. 8, 2019, previously published as PCT/EP2016/050468, filed on Jan. 12, 2016.
Claims priority of application No. 15150819 (EP), filed on Jan. 12, 2015.
Prior Publication US 2021/0052713 A1, Feb. 25, 2021
Int. Cl. A61K 39/108 (2006.01); A61K 38/03 (2006.01); A61K 38/16 (2006.01); C07K 14/26 (2006.01); C07K 16/12 (2006.01); C12N 15/70 (2006.01); C12N 15/75 (2006.01); C12N 15/79 (2006.01); G01N 33/569 (2006.01)
CPC A61K 39/0266 (2013.01) [A61K 38/03 (2013.01); A61K 38/164 (2013.01); C07K 14/26 (2013.01); C07K 16/12 (2013.01); C12N 15/79 (2013.01); G01N 33/56916 (2013.01); C07K 2317/24 (2013.01); C07K 2317/54 (2013.01); C07K 2317/55 (2013.01); C07K 2317/622 (2013.01); C12N 15/70 (2013.01); C12N 15/75 (2013.01); G01N 2333/26 (2013.01); G01N 2469/20 (2013.01)] 19 Claims
 
1. A method for treatment or amelioration of infection with K. pneumoniae, comprising administering to an individual in need thereof a therapeutically effective amount of a monoclonal antibody, which specifically binds to a polypeptide consisting of
a) an amino acid sequence SEQ ID NO: 25, or
b) an amino acid sequence, which is a fragment of SEQ ID NO: 25 consisting of residues 35-790 or residues 450-790 or residues 35-450,
wherein, the monoclonal antibody is obtained by immunizing an animal with a protein comprising SEQ ID NO:25 or residues 35-790 or residues 450-790 of SEQ ID NO:25, isolating B-cells that bind SEQ ID NO: 25 or residues 35-790 or residues 450-790 of SEQ ID NO:25 from the animal, and using said B-cells in the production of the monoclonal antibody, which in useful in conferring passive immunity against K. pneumoniae in the individual.