US 12,226,437 B2
Method for enhancing expression of insulin like growth factor 1 receptor in umbilical cord mesenchymal stem cell expressing IGF1R and method for obtaining multipotent umbilical cord mesenchymal stem cell
Woei-Cherng Shyu, Taichung (TW); Chen-Huan Lin, Taichung (TW); Wei Lee, Taipei (TW); Chia-Hung Hsieh, Taichung (TW); Chung-Y. Hsu, Taichung (TW); and Chang-Hai Tsai, Taichung (TW)
Assigned to China Medical University, Taichung (TW)
Filed by China Medical University, Taichung (TW)
Filed on Nov. 7, 2022, as Appl. No. 17/981,736.
Application 17/981,736 is a division of application No. 16/835,771, filed on Mar. 31, 2020, granted, now 11,529,373.
Application 16/835,771 is a division of application No. 15/853,989, filed on Dec. 26, 2017, granted, now 10,660,918, issued on May 26, 2020.
Application 15/853,989 is a division of application No. 14/934,162, filed on Nov. 6, 2015, abandoned.
Claims priority of application No. 104123719 (TW), filed on Jul. 22, 2015.
Prior Publication US 2023/0056048 A1, Feb. 23, 2023
Int. Cl. A61K 35/28 (2015.01); A61K 38/17 (2006.01); A61K 38/18 (2006.01); A61P 25/28 (2006.01); C12N 5/073 (2010.01); C12N 5/0775 (2010.01); A61K 35/12 (2015.01); A61K 45/06 (2006.01)
CPC A61K 35/28 (2013.01) [A61K 38/179 (2013.01); A61K 38/1858 (2013.01); A61P 25/28 (2018.01); C12N 5/0605 (2013.01); C12N 5/0665 (2013.01); C12N 5/0668 (2013.01); A61K 2035/124 (2013.01); A61K 45/06 (2013.01); C12N 2500/84 (2013.01)] 4 Claims
 
1. A method for enhancing an expression of insulin like growth factor 1 receptor (IGF1R) in an umbilical cord mesenchymal stem cell expressing IGF1R, the method comprising:
deriving a plurality of umbilical cord mesenchymal stem cells from a Wharton's jelly;
isolating the umbilical cord mesenchymal stem cell expressing IGF1R from the plurality of umbilical cord mesenchymal stem cells by using an IGF1R antibody; and
culturing the umbilical cord mesenchymal stem cell expressing IGF1R in a medium containing platelet-derived growth factor BB (PDGF-BB) at 2.5-50 ng/ml to enhance the expression of IGF1R in the umbilical cord mesenchymal stem cell expressing IGF1R.