	US 12,226,430 B2
	Altering microbial populations and modifying microbiota
Jasper Clube, London (GB); Morten Sommer, London (GB); Christian Grøndahl, London (GB); Eric Van Der Helm, London (GB); and Ruben Vazquez-Uribe, London (GB)
Assigned to SNIPR TECHNOLOGIES LIMITED, Peterborough (GB)
Filed by SNIPR Technologies Limited, Peterborough (GB)
Filed on Nov. 3, 2023, as Appl. No. 18/501,825.
Application 16/813,615 is a division of application No. 15/460,962, filed on Mar. 16, 2017, granted, now 10,582,712, issued on Mar. 10, 2020.
Application 18/501,825 is a continuation of application No. 16/813,615, filed on Mar. 9, 2020, granted, now 11,844,760.
Application 15/460,962 is a continuation of application No. 15/160,405, filed on May 20, 2016, granted, now 9,701,964, issued on Jul. 11, 2017.
Application 15/160,405 is a continuation of application No. PCT/EP2016/059803, filed on May 3, 2016.
Claims priority of application No. 1507773.8 (GB), filed on May 6, 2015; application No. 1507774.6 (GB), filed on May 6, 2015; application No. 1507775.3 (GB), filed on May 6, 2015; application No. 1507776.1 (GB), filed on May 6, 2015; application No. 1508461.9 (GB), filed on May 17, 2015; application No. 1509366.9 (GB), filed on May 31, 2015; application No. 1510891.3 (GB), filed on Jun. 20, 2015; application No. 1518402.1 (GB), filed on Oct. 17, 2015; application No. 1600417.8 (GB), filed on Jan. 10, 2016; and application No. 1600418.6 (GB), filed on Jan. 10, 2016.
Prior Publication US 2024/0082289 A1, Mar. 14, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 31/711 (2006.01); A01N 63/00 (2020.01); A01N 63/50 (2020.01); A01N 63/60 (2020.01); A61K 31/7105 (2006.01); A61K 35/74 (2015.01); A61K 45/06 (2006.01); A61K 48/00 (2006.01); C12N 1/20 (2006.01); C12N 7/00 (2006.01); C12N 9/16 (2006.01); C12N 15/10 (2006.01); C12N 15/113 (2010.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01); C12N 15/90 (2006.01); A61K 35/00 (2006.01)

CPC A61K 31/7105 (2013.01) [A01N 63/00 (2013.01); A01N 63/50 (2020.01); A01N 63/60 (2020.01); A61K 31/711 (2013.01); A61K 35/74 (2013.01); A61K 45/06 (2013.01); A61K 48/005 (2013.01); C12N 1/20 (2013.01); C12N 7/00 (2013.01); C12N 9/16 (2013.01); C12N 15/102 (2013.01); C12N 15/113 (2013.01); C12N 15/70 (2013.01); C12N 15/746 (2013.01); C12N 15/902 (2013.01); A61K 2035/11 (2013.01); A61K 2300/00 (2013.01); C12N 2310/20 (2017.05); C12N 2320/31 (2013.01); C12N 2795/00032 (2013.01); C12N 2795/10132 (2013.01); Y02A 50/30 (2018.01)]

16 Claims

1. A method of modifying a mixed population of bacteria, wherein the mixed population comprises a first bacterial sub-population and a second bacterial sub-population, wherein the first bacterial sub-population comprises target host cells of a first bacterial species and the second bacterial sub-population comprises second cells of a second bacterial species, wherein the first bacterial species is different from the second bacterial species, wherein the second cells comprise a conjugative plasmid comprising an origin of transfer (oriT) and a nucleic acid sequence for producing a CRISPR RNA (crRNA), and wherein the method comprises:

a. transferring the conjugative plasmid from second cells of the second bacterial sub-population to the target host cells, and

b. producing the crRNA in the target host cells,

wherein the host cells comprise an exogenous nucleotide sequence for producing a desired protein in the host cell,

wherein the nucleic acid sequence for producing the crRNA is under control of an inducible promoter in the host cell, and

wherein the crRNA comprises a nucleic acid sequence that is capable of hybridizing to a target nucleotide sequence in the target host cells to guide a Cas to modify the target nucleotide sequence in the target host cells, wherein the modification of the target sequence inhibits expression of the desired protein in the host cell.