US 12,226,186 B2
Devices, methods, and systems with spectral filtering for detecting wound and identifying bacteria based on fluorescence signature
Ralph Sebastian Dacosta, Etobicoke (CA); Brian C. Wilson, Toronto (CA); and Kai Zhang, Scarborough (CA)
Assigned to UNIVERSITY HEALTH NETWORK, Toronto (CA)
Filed by UNIVERSITY HEALTH NETWORK, Toronto (CA)
Filed on Mar. 5, 2021, as Appl. No. 17/193,318.
Application 17/193,318 is a continuation of application No. 14/719,493, filed on May 22, 2015, granted, now 11,375,898.
Application 14/719,493 is a continuation of application No. 12/992,040, granted, now 9,042,967, issued on May 26, 2015, previously published as PCT/CA2009/000680, filed on May 20, 2009.
Claims priority of provisional application 61/054,780, filed on May 20, 2008.
Prior Publication US 2021/0259552 A1, Aug. 26, 2021
Int. Cl. A61B 5/00 (2006.01); A61B 5/01 (2006.01); A61B 10/00 (2006.01); G01N 21/64 (2006.01)
CPC A61B 5/0071 (2013.01) [A61B 5/0059 (2013.01); A61B 5/01 (2013.01); A61B 5/445 (2013.01); A61B 5/72 (2013.01); A61B 5/742 (2013.01); G01N 21/6456 (2013.01); G01N 21/6486 (2013.01); A61B 5/0042 (2013.01); A61B 5/4519 (2013.01); A61B 10/00 (2013.01); G01N 21/6408 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6471 (2013.01); G01N 2201/0221 (2013.01); Y02A 90/10 (2018.01)] 20 Claims
OG exemplary drawing
 
1. A handheld, portable imaging system for acquiring data regarding a wound in tissue, comprising:
at least one excitation light source configured to illuminate a wound with excitation light and comprising at least one wavelength or wavelength band causing at least one biomarker in the illuminated wound to fluoresce;
a spectral filtering mechanism configured to permit passage of optical signals responsive to illumination of the wound and having a wavelength corresponding to bacterial autofluorescence and/or bacterial fluorescence, the spectral filtering mechanism including a plurality of selectable filters respectively corresponding to different discrete spectral bandwidths;
a mobile communication device having an image sensor to detect the spectrally filtered signals, wherein the mobile communication device is a cellular telephone, a smartphone, a laptop computer, a tablet computer, or a personal digital assistant,
wherein the plurality of selectable filters is movable relative to the image sensor so as to position a selected filter of the plurality of selectable filters between the wound and the image sensor to detect the spectrally filtered signals corresponding to the discrete spectral bandwidths of the selected filter;
a housing, separate from the mobile communication device, to support the at least one excitation light source and the spectral filtering mechanism relative to the mobile communication device during imaging and configured to receive at least a portion of the mobile communication device therein; wherein the spectral filtering mechanism includes a triple band-pass filter; and
a processor configured to receive the detected, filtered signals, to spatially and/or temporally co-register one or more of endogenous fluorescence data, exogenous fluorescence data, absorbance data, and reflectance data contained in the detected, filtered signals, and to identify a fluorescent signature of bacteria in the wound based at least in part on the data contained in the detected, filtered signals, and to output data regarding the bacterial fluorescent signature.