	US 12,226,158 B2
	Systems for off-axis imaging of a surface of a sample and related methods and computer program products
Robert H. Hart, Cary, NC (US); Dorothy M. Branco, Durham, NC (US); Eric L. Buckland, Hickory, NC (US); and Micaela R. Mendlow, Jersey City, NJ (US)
Assigned to LEICA MICROSYSTEMS NC, INC., Durham, NC (US)
Appl. No. 17/424,056
Filed by Leica Microsystems NC, Inc., Durham, NC (US)
PCT Filed Mar. 19, 2020, PCT No. PCT/US2020/023544 § 371(c)(1), (2) Date Jul. 19, 2021, PCT Pub. No. WO2020/191148, PCT Pub. Date Sep. 24, 2020.
Claims priority of provisional application 62/821,556, filed on Mar. 21, 2019.
Prior Publication US 2022/0125301 A1, Apr. 28, 2022
Int. Cl. A61B 3/10 (2006.01); A61B 3/00 (2006.01); G06T 7/33 (2017.01)

CPC A61B 3/102 (2013.01) [A61B 3/0025 (2013.01); G06T 7/344 (2017.01); G06T 2207/10101 (2013.01); G06T 2207/30041 (2013.01)]

20 Claims

1. A system for determining an apex of curvature in an image obtained from a sample, the system comprising:

an imaging system configured to obtain a plurality of scans of a sample; and

a processor associated with the imaging system, the processor configured to:

segment and curve fit each respective scan of the plurality of scans to a surface of the sample to determine a respective apex, wherein each respective scan is along a respective radial line at a respective angle in a polar coordinate system, an origin of the polar coordinate system being off of a corneal axis;

determine a true apex among the determined apexes using a derivative of least value;

calculate an XY offset based on the determined true apex;

map the true apex to an origin where X and Y are equal to zero; and

adjust the coordinates associated with remaining apexes not-determined to be not the true apex based on the calculated offset.