US 12,225,889 B2
Pathogen-resistant animals having modified CD163 genes
Randall S. Prather, Rocheport, MO (US); Kevin D. Wells, Columbia, MO (US); and Kristin M. Whitworth, Columbia, MO (US)
Assigned to The Curators of the University of Missouri, Columbia, MO (US)
Filed by The Curators of the University of Missouri, Columbia, MO (US)
Filed on Nov. 30, 2023, as Appl. No. 18/524,212.
Application 18/524,212 is a continuation of application No. 17/036,522, filed on Sep. 29, 2020.
Application 17/036,522 is a continuation of application No. 15/750,633, granted, now 10,827,730, issued on Nov. 10, 2020, previously published as PCT/US2016/043467, filed on Jul. 22, 2016.
Claims priority of provisional application 62/202,145, filed on Aug. 6, 2015.
Prior Publication US 2024/0122164 A1, Apr. 18, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/0276 (2024.01); A01K 67/02 (2006.01); C07K 14/705 (2006.01); C12N 5/075 (2010.01); C12N 5/076 (2010.01); C12N 5/10 (2006.01); C12N 9/22 (2006.01); C12N 15/09 (2006.01); C12N 15/85 (2006.01); C12N 15/877 (2010.01)
CPC A01K 67/0276 (2013.01) [A01K 67/02 (2013.01); C07K 14/70596 (2013.01); C12N 5/0609 (2013.01); C12N 5/061 (2013.01); C12N 5/10 (2013.01); C12N 9/22 (2013.01); C12N 15/09 (2013.01); C12N 15/8509 (2013.01); C12N 15/8778 (2013.01); A01K 2217/075 (2013.01); A01K 2227/108 (2013.01); A01K 2267/02 (2013.01); C12N 2310/20 (2017.05); C12N 2770/10011 (2013.01)] 18 Claims
 
1. A method of increasing porcine reproductive and respiratory (PRRS) resistance in a porcine animal, offspring, or cell, relative to a wild-type porcine animal, offspring, or cell, comprising:
modifying at least one chromosomal sequence in a gene encoding a CD163 protein in the porcine animal, offspring, or cell, wherein the modification is selected from the group consisting of:
a modification in exon 7 of the gene encoding the CD163 protein,
a modification in exon 8 of the gene encoding the CD163 protein,
a modification in an intron that is contiguous with exon 7 or exon 8 of the gene encoding the CD163 protein, and
a combination thereof, wherein the modification is selected from the group consisting of
an 11 bp deletion from nucleotide 3137 to nucleotide 3147 relative to the reference sequence SEQ ID NO:47,
a 377 bp deletion from nucleotide 2573 to nucleotide 2949, with a 2 bp addition between nucleotides 3149 to nucleotide 3150 relative to the reference sequence SEQ ID NO:47 on the same allele,
a 1930 base pair deletion from nucleotide 488 to nucleotide 2,417, wherein the deleted sequence is replaced with a 12 base pair insertion beginning at nucleotide 488, with a 129 base pair deletion from nucleotide 3044 to nucleotide 3172 relative to the reference sequence SEQ ID NO: 47 on the same allele,
a 1467 bp deletion from nucleotide 2,431 to nucleotide 3,897 relative to the reference sequence SEQ ID NO:47,
a 1280 bp deletion from nucleotide 2818 to nucleotide 4097 relative to the reference sequence SEQ ID NO:47, and
combinations thereof.