	US 12,225,888 B2
	Transgenic rainbow shark
Alan Blake, Austin, TX (US); Richard Crockett, Wilton, CT (US); and Aidas Nasevicius, Tampa, FL (US)
Assigned to GLOFISH, LLC, Earth City, MO (US)
Appl. No. 16/961,195
Filed by GloFish, LLC, Earth City, MO (US)
PCT Filed Jan. 10, 2019, PCT No. PCT/US2019/013072 § 371(c)(1), (2) Date Jul. 9, 2020, PCT Pub. No. WO2019/140103, PCT Pub. Date Jul. 18, 2019.
Claims priority of provisional application 62/615,638, filed on Jan. 10, 2018.
Claims priority of provisional application 62/615,634, filed on Jan. 10, 2018.
Claims priority of provisional application 62/615,628, filed on Jan. 10, 2018.
Claims priority of provisional application 62/615,625, filed on Jan. 10, 2018.
Prior Publication US 2021/0051927 A1, Feb. 25, 2021
Int. Cl. A01K 67/027 (2024.01); A01K 67/0275 (2024.01); C12N 15/85 (2006.01)

CPC A01K 67/0275 (2013.01) [C12N 15/8509 (2013.01); A01K 2217/00 (2013.01); A01K 2227/40 (2013.01); A01K 2267/01 (2013.01)]

9 Claims

1. A transgenic rainbow shark sperm whose genome comprises:

a) an exogenous nucleic sequence encoding an orange fluorescent protein, wherein the transgenic rainbow shark sperm is deposited at the European Collection of Authenticated Cell Cultures (ECACC) as Accession Number 19010802;

b) an exogenous nucleic sequence encoding a purple fluorescent protein, wherein the transgenic rainbow shark sperm is deposited at the ECACC as Accession Number 19010801;

c) an exogenous nucleic sequence encoding a blue fluorescent protein, wherein the transgenic rainbow shark sperm is deposited at the ECACC as Accession Number 19010803; or

d) an exogenous nucleic sequence encoding a green fluorescent protein, wherein the transgenic rainbow shark sperm is deposited at the ECACC as Accession Number 19010804.