US 11,899,015 B1
Chimeric recombinant proteins and recombinant protein panels for the diagnosis of Lyme disease in animals and humans
Richard Marconi, Richmond, VA (US)
Assigned to VIRGINIA COMMONWEALTH UNIVERSITY, Richmond, VA (US)
Filed by VIRGINIA COMMONWEALTH UNIVERSITY, Richmond, VA (US)
Filed on Oct. 24, 2022, as Appl. No. 18/049,022.
Int. Cl. G01N 33/569 (2006.01); C12Q 1/6883 (2018.01); C12Q 1/689 (2018.01); C07K 14/20 (2006.01)
CPC G01N 33/56911 (2013.01) [C07K 14/20 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6883 (2013.01); C07K 2319/21 (2013.01); G01N 2333/20 (2013.01)] 3 Claims
 
1. A recombinant chimeric polypeptide comprising, in order from amino to carboxy terminus:
i) DbpB without N terminal A (SEQ ID NO: 76) or a variant thereof having at least 95% identity to SEQ ID NO: 76,
BBA73 (SEQ ID NO: 3) or a variant thereof having at least 95% identity to SEQ ID NO: 73,
BBK53 (SEQ ID NO: 4) or a variant thereof having at least 95% identity to SEQ ID NO: 4, and
BBO238 (SEQ ID NO: 5) or a variant thereof having at least 95% identity to SEQ ID NO: 5;
or
ii) DbpB without N terminal A (SEQ ID NO: 76) or a variant thereof having at least 95% identity to SEQ ID NO:76,
BBA73 (SEQ ID NO: 3) or a variant thereof having at least 95% identity to SEQ ID NO: 3, and
BBK53 (SEQ ID NO: 4) or a variant thereof having at least 95% identity to SEQ ID NO:4;
or
iii) BBA24 (SEQ ID NO: 13) or a variant thereof having at least 95% identity to SEQ ID NO:13,
DbpB (SEQ ID NO: 2) or a variant thereof having at least 95% identity to SEQ ID NO: 2,
BBK19 (SEQ ID NO: 15) or a variant thereof having at least 95% identity to SEQ ID NO: 15, and
VlsE (SEQ ID NO: 16) or a variant thereof having at least 95% identity to SEQ ID NO:16.